Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry

Chen, Zhen; Wu, Yue; Shrestha, Rojeet; Gao, Zijun; Zhao, Yaoyao; Miura, Yusuke; Tamakoshi, Akiko; Chiba, Hitoshi; Hui, Shu-Ping

Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry

Chen, Zhen; Wu, Yue; Shrestha, Rojeet et al.

Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 2019, 56(2), 190-197

Permalink : https://hdl.handle.net/2115/76142

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アクセス権	open access
URI	https://hdl.handle.net/2115/76142
タイトル	Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry
著者	Chen, Zhen ; Wu, Yue ; Shrestha, Rojeet ; Gao, Zijun ; Zhao, Yaoyao ; Miura, Yusuke ; Tamakoshi, Akiko ; Chiba, Hitoshi ; Hui, Shu-Ping
著者名 Chen, Zhen
著者名 Wu, Yue
著者名 Shrestha, Rojeet
著者名 Gao, Zijun
著者名 Zhao, Yaoyao
著者名 Miura, Yusuke
e-Rad_Researcher 90236737 著者名 Tamakoshi, Akiko
e-Rad_Researcher 70197622 著者名 Chiba, Hitoshi
e-Rad_Researcher 90337030 著者名 Hui, Shu-Ping
言語	英語
キーワード	Short-chain fatty acid
	butanoic acid (FA 4:0)
	caproic acid (FA 6:0)
	LC-MS/MS
	serum
発行日	2019-03
出版者	Sage
収録物名	Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
巻(号)	56(2)
ページ	190-197
抄録	BACKGROUND:Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. METHODS:Blood samples from human subjects ( n = 547, male/female = 246/301, age 58.85 ± 12.57) were collected. Saponification was applied to obtain total fatty acid. After derivatization by 2-nitrophenylhydrazine, fatty acid 4:0 and fatty acid 6:0 were measured by liquid chromatography-mass spectrometry. RESULTS:The developed method exhibited good linearity (R2 = 0.9996 for both). All the coefficients of variation of reproducibility and accuracy for fatty acid 4:0 and fatty acid 6:0 ranged 3.0%-6.1%, with the average recoveries of 87.8%-102.4% and 92.2%-98.2%, respectively. In all the samples, the concentration of fatty acid 4:0 (162.4 ± 76.4 μmol/L) was significantly higher than fatty acid 6:0 (2.0 ± 2.5 μmol/L, P < 0.001). Furthermore, the esterified form was predominant in both fatty acid 4:0 and fatty acid 6:0 (98.2% and 82.4% of total fatty acids, respectively). Besides, short-chain fatty acids showed no significant differences with regard to sex or age differences. CONCLUSION:This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Chen Zhen, Yue Wu, Rojeet Shrestha, Zijun Gao, Yaoyao Zhao, Yusuke Miura, Akiko Tamakoshi, Hitoshi Chiba, and Shu-Ping Hui, Determination of Total, Free and Esterified Short-Chain Fatty Acid in Human Serum by Liquid Chromatography-Mass Spectrometry, Annals of Clinical Biochemistry (v.56, no. 2): pp. 190–97. Copyright © 2019 (SAGE). Reprinted by permission of SAGE Publications. DOI: 10.1177/0004563218801393.
PISSN	0004-5632
関連情報	PMID 30185055
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1177/0004563218801393