Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage

Mendis, Charitha; Thevanesam, Vasanthi; Kumara, Athula; Wickramasinghe, Susiji; Madegedara, Dushantha; Gamage, Chandika; Gordon, Stephen V.; Suzuki, Yasuhiko; Ratnatunga, Champa; Nakajima, Chie

Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage

Mendis, Charitha; Thevanesam, Vasanthi; Kumara, Athula et al.

International journal of infectious diseases, 2019, 87, 84-91

Permalink : https://hdl.handle.net/2115/76196

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アクセス権	open access
URI	https://hdl.handle.net/2115/76196
タイトル	Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage
著者	Mendis, Charitha ; Thevanesam, Vasanthi ; Kumara, Athula ; Wickramasinghe, Susiji ; Madegedara, Dushantha ; Gamage, Chandika ; Gordon, Stephen V. ; Suzuki, Yasuhiko ; Ratnatunga, Champa ; Nakajima, Chie
著者名 Mendis, Charitha
著者名 Thevanesam, Vasanthi
著者名 Kumara, Athula
著者名 Wickramasinghe, Susiji
著者名 Madegedara, Dushantha
著者名 Gamage, Chandika
著者名 Gordon, Stephen V.
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
著者名 Ratnatunga, Champa
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
言語	英語
キーワード	Mycobacterium tuberculosis
	Spoligotype
	MIRU-VNTR
	Lineage 4 (Euro-American lineage)
	Sri Lanka
発行日	2019-10
出版者	Elsevier
収録物名	International journal of infectious diseases
巻(号)	87
ページ	84-91
抄録	Objective: Sri Lanka is a country where the molecular epidemiology of Mycobacterium tuberculosis (MTB) is poorly explored. Therefore, this study was performed to identify circulating lineages/sub-lineages of MTB and their transmission patterns. Methods: DNA was extracted from 89 isolates of MTB collected during 2012 and 2013 from new pulmonary tuberculosis patients in Kandy, Sri Lanka and analyzed by spoligotyping, large sequence polymorphism (LSP), mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing, and drug resistance-associated gene sequencing. Results: The predominant lineage was lineage 4 (Euro-American, 45.9%), followed by lineage 1 (Indo-Oceanic, 29.4%), lineage 2 (East-Asian, 23.5%), and lineage 3 (Central-Asian, 1.2%). Among 26 spoligotype patterns, eight were undesignated or new types and seven of these belonged to lineage 4. Undesignated lineage 4/SIT124 (n = 2/8) and SIT3234 (n = 8/8) clustered together based on 24-locus MIRU-VNTR typing. The dominant sub-lineage was Beijing/SIT1 (n = 19), with the isoniazid resistance katG G944C mutation (Ser315Thr) detected in two of them. Conclusions: The population structure of MTB in Kandy, Sri Lanka was different from that in the South Asian region. The clonal expansion of locally evolved lineage 4/SIT3234 and detection of the premultidrug resistant Beijing isolates from new tuberculosis patients is alarming and will require continuous monitoring. (C) 2019 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	1201-9712
EISSN	1878-3511
NCID	AA11238338
関連情報	PMID 31299365
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.ijid.2019.07.001