Comparison of xenobiotic metabolism in phase I oxidation and phase II conjugation between rats and bird species

Saengtienchai, Aksorn; Ikenaka, Yoshinori; Kawata, Minami; Kawai, Yusuke; Takeda, Kazuki; Kondo, Takamitsu; Bortey-Sam, Nesta; Nakayama, Shouta M. M.; Mizukawa, Hazuki; Ishizuka, Mayumi

Comparison of xenobiotic metabolism in phase I oxidation and phase II conjugation between rats and bird species

Saengtienchai, Aksorn; Ikenaka, Yoshinori; Kawata, Minami et al.

Comparative biochemistry and physiology Part C: Toxicology & pharmacology, 2018, 214, 28-35

Permalink : https://hdl.handle.net/2115/76244

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アクセス権	open access
URI	https://hdl.handle.net/2115/76244
タイトル	Comparison of xenobiotic metabolism in phase I oxidation and phase II conjugation between rats and bird species
著者	Saengtienchai, Aksorn ; Ikenaka, Yoshinori ; Kawata, Minami ; Kawai, Yusuke ; Takeda, Kazuki ; Kondo, Takamitsu ; Bortey-Sam, Nesta ; Nakayama, Shouta M. M. ; Mizukawa, Hazuki ; Ishizuka, Mayumi
著者名 Saengtienchai, Aksorn
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
著者名 Kawata, Minami
著者名 Kawai, Yusuke
著者名 Takeda, Kazuki
著者名 Kondo, Takamitsu
著者名 Bortey-Sam, Nesta
著者名 Nakayama, Shouta M. M.
e-Rad_Researcher 60612661 著者名 Mizukawa, Hazuki
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	Birds
	Species differences
	Pyrene
	Conjugated metabolites
	Kinetics
発行日	2018-12
出版者	Elsevier
収録物名	Comparative biochemistry and physiology Part C: Toxicology & pharmacology
巻(号)	214
ページ	28-35
抄録	There have been many reports regarding toxic chemicals in birds. Chemicals are mainly metabolized in the liver through phase I oxidation by cytochrome P450 (GYP) and phase II conjugation by conjugated enzymes, such as UDP-glucuronosyltransferase (UGT), sulfotransferase (SULT), glutathione-S-transferase (GST), etc. Xenobiotic metabolism differs among bird species, but little detailed information is available. In the present study, the four ring polycyclic aromatic hydrocarbon (PAH), pyrene, was used as a model xenobiotic to clarify the characteristics of xenobiotic metabolism in birds compared with laboratory animals by in vivo and in vitro studies. Plasma, bile, and excreta (urine and feces) were collected after oral administration of pyrene and analyzed to clarify xenobiotic metabolism ability in chickens and quails. Interestingly, pyrenediol-glucuronide sulfate (PYDOGS) and pyrenediol-diglucuronide (PYDOGG) were present in chickens and quails but not in rats. In addition, the area under the curve (AUC), maximum plasma concentration (C-max), and time to maximum plasma concentration (T-max) of pyrene-1-sulfate (PYOS) were higher than those of the parent molecule, pyrene, while the elimination half-life (t(1/2)) and mean residence time (MRT) were faster than those of the parent pyrene. With regard to sulfation of 1-hydroxypyrene (PYOH), the maximum velocity (V-max) and Michaelis constant (K-m) of rat liver cytosol were greater than those of chicken and quail liver cytosol. Furthermore, V-max/K-m of UGT activity in rat liver microsomes was also greater than those of chicken and quail liver microsomes. Characterization of xenobiotic metabolism revealed species differences between birds and mammals, raising concerns about exposure to various xenobiotics in the environment.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1532-0456
関連情報	PMID 30176376
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.cbpc.2018.08.007