Unsaturated Aldehyde Acrolein Promotes Retinal Glial Cell Migration

Murata, Miyuki; Noda, Kousuke; Yoshida, Shiho; Saito, Michiyuki; Fujiya, Akio; Kanda, Atsuhiro; Ishida, Susumu

Unsaturated Aldehyde Acrolein Promotes Retinal Glial Cell Migration

Murata, Miyuki; Noda, Kousuke; Yoshida, Shiho et al.

Investigative ophthalmology & visual science, 2019, 60(13), 4425-4435

Permalink : https://hdl.handle.net/2115/76301

このアイテムのアクセス数:647件（2026-08-25 04:16 集計）

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https://doi.org/10.1167/iovs.19-27346			35

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/76301
タイトル	Unsaturated Aldehyde Acrolein Promotes Retinal Glial Cell Migration
著者	Murata, Miyuki ; Noda, Kousuke ; Yoshida, Shiho ; Saito, Michiyuki ; Fujiya, Akio ; Kanda, Atsuhiro ; Ishida, Susumu
e-Rad_Researcher 50423752 著者名 Murata, Miyuki
e-Rad_Researcher 90296666 著者名 Noda, Kousuke
著者名 Yoshida, Shiho
著者名 Saito, Michiyuki
著者名 Fujiya, Akio
e-Rad_Researcher 80342707 著者名 Kanda, Atsuhiro
e-Rad_Researcher 10245558 著者名 Ishida, Susumu
言語	英語
キーワード	Acrolein
	CXCL1
	oxidative stress
	Muller cell
発行日	2019-10
出版者	Association for Research in Vision and Ophthalmology（ARVO）
収録物名	Investigative ophthalmology & visual science
巻(号)	60(13)
ページ	4425-4435
抄録	PURPOSE. To investigate the effect of the unsaturated aldehyde acrolein on retinal glial cell migration. METHODS. Muller glial cell markers expression in TR-MUL5 were confirmed by RT-PCR and immunostaining. Cell viability and migration rate of TR-MUL5 cells were assessed after the stimulation with acrolein. DNA microarray analysis was performed to analyze changes in the expression levels of migration-related genes in Muller glial cells stimulated with acrolein. Real-time PCR and ELISA were performed to validate DNA microarray analysis results. Inhibitors of C-X-C motif chemokine ligand 1 (CXCL1), one of the genes highly upregulated after the exposure to acrolein, and blockers of its receptor, CXCR2, were used to investigate the role of the CXCL1-CXCR2 axis on glial cell migration. CXCL1 concentration was measured in vitreous fluid samples obtained from proliferative diabetic retinopathy (PDR) and nondiabetic control eyes. CXCL1 and CXCR2 expression in glial cells of fibrovascular tissues obtained from PDR patients was examined by immunostaining. RESULTS. At a high concentration, acrolein (100 mu M) significantly decreased cell viability. However, in moderate, sublethal concentrations (25-50 mu M), acrolein induced cell migration and substantially increased the production of CXCL1 in TR-MUL5 cells. CXCL1 concentration was significantly elevated in vitreous fluids of PDR patients, and CXCL1 and CXCR2 were present in glial cells in fibrovascular tissues of PDR patients. CXCL1 stimulation increased glial cell migration in a dose-dependent manner, which was abrogated by the neutralization of the CXCL1-CXCR2 axis. CONCLUSIONS. Our data demonstrate that acrolein promotes retinal Muller glial cell migration by enhancing CXCL1 production.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0146-0404
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1167/iovs.19-27346