Single-nucleotide polymorphisms in human NPC1 influence filovirus entry into cells

Kondoh, Tatsunari; Letko, Michael; Munster, Vincent J.; Manzoor, Rashid; Maruyama, Junki; Furuyama, Wakako; Miyamoto, Hiroko; Shigeno, Asako; Fujikura, Daisuke; Takadate, Yoshihiro; Yoshida, Reiko; Igarashi, Manabu; Feldmann, Heinz; Marzi, Andrea; Takada, Ayato

Single-nucleotide polymorphisms in human NPC1 influence filovirus entry into cells

Kondoh, Tatsunari; Letko, Michael; Munster, Vincent J. et al.

Journal of Infectious Diseases, 2018, 218(suppl 5), S397-S402

Permalink : https://hdl.handle.net/2115/76336

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アクセス権	open access
URI	https://hdl.handle.net/2115/76336
タイトル	Single-nucleotide polymorphisms in human NPC1 influence filovirus entry into cells
著者	Kondoh, Tatsunari ; Letko, Michael ; Munster, Vincent J. ; Manzoor, Rashid ; Maruyama, Junki ; Furuyama, Wakako ; Miyamoto, Hiroko ; Shigeno, Asako ; Fujikura, Daisuke ; Takadate, Yoshihiro ; Yoshida, Reiko ; Igarashi, Manabu ; Feldmann, Heinz ; Marzi, Andrea ; Takada, Ayato
著者名 Kondoh, Tatsunari
著者名 Letko, Michael
著者名 Munster, Vincent J.
著者名 Manzoor, Rashid
著者名 Maruyama, Junki
著者名 Furuyama, Wakako
著者名 Miyamoto, Hiroko
著者名 Shigeno, Asako
e-Rad_Researcher 70547794 著者名 Fujikura, Daisuke
著者名 Takadate, Yoshihiro
e-Rad_Researcher 80435966 著者名 Yoshida, Reiko
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
著者名 Feldmann, Heinz
著者名 Marzi, Andrea
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
キーワード	Ebolavirus
	Marburgvirus
	Niemann-Pick C1 (NPC1)
	Single-nucleotide polymorphism (SNP)
	NPC1-knockout Vero E6 (Vero E6/NPC1-KO)
発行日	2018-12-15
出版者	Oxford University Press
収録物名	Journal of Infectious Diseases
巻(号)	218(suppl 5)
ページ	S397-S402
抄録	Niemann-Pick C1 (NPC1), a host receptor involved in the envelope glycoprotein (GP)-mediated entry of filoviruses into cells, is believed to be a major determinant of cell susceptibility to filovirus infection. It is known that proteolytically digested Ebola virus (EBOV) GP interacts with 2 protruding loops in domain C of NPC1. Using previously published structural data and the National Center for Biotechnology Information Single-Nucleotide Polymorphism (SNP) database, we identified 10 naturally occurring missense SNPs in human NPC1. To investigate whether these SNPs affect cell susceptibility to filovirus infection, we generated Vero E6 cell lines stably expressing NPC1 with SNP substitutions and compared their susceptibility to vesicular stomatitis virus pseudotyped with filovirus GPs and infectious EBOV. We found that some of the substitutions resulted in reduced susceptibility to filoviruses, as indicated by the lower titers and smaller plaque/focus sizes of the viruses. Our data suggest that human NPC1 SNPs may likely affect host susceptibility to filoviruses.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Infectious Diseases following peer review. The version of record Volume 218 Issue suppl_5, S397–S402, 2018 is available online at: https://doi.org/10.1093/infdis/jiy248.
PISSN	0022-1899
EISSN	1537-6613
NCID	AA0070010X
関連情報	PMID 30010949
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/infdis/jiy248