Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)

Paudel, Sarad; Villanueva, A. Marvin; Mikota, K. Susan; Nakajima, Chie; Gairhe, P. Kamal; Subedi, Suraj; Rayamajhi, Nabin; Sashika, Mariko; Shimozuru, Michito; Matsuba, Takashi; Suzuki, Yasuhiko; Tsubota, Toshio

Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)

Paudel, Sarad; Villanueva, A. Marvin; Mikota, K. Susan et al.

Journal of Veterinary Medical Science, 2016, 78(7), 1117-1121

Permalink : https://hdl.handle.net/2115/76354

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アクセス権	open access
URI	https://hdl.handle.net/2115/76354
タイトル	Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)
著者	Paudel, Sarad ; Villanueva, A. Marvin ; Mikota, K. Susan ; Nakajima, Chie ; Gairhe, P. Kamal ; Subedi, Suraj ; Rayamajhi, Nabin ; Sashika, Mariko ; Shimozuru, Michito ; Matsuba, Takashi ; Suzuki, Yasuhiko ; Tsubota, Toshio
e-Rad_Researcher 30793939 著者名 Paudel, Sarad
著者名 Villanueva, A. Marvin
著者名 Mikota, K. Susan
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
著者名 Gairhe, P. Kamal
著者名 Subedi, Suraj
著者名 Rayamajhi, Nabin
著者名 Sashika, Mariko
e-Rad_Researcher 50507168 著者名 Shimozuru, Michito
e-Rad_Researcher 20304206 著者名 Matsuba, Takashi
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
e-Rad_Researcher 10207441 著者名 Tsubota, Toshio
言語	英語
キーワード	Asian elephant
	interferon-γ
	mitogen
	sandwich ELISA
発行日	2016-07
出版者	The Japanese Society of Veterinary Science
出版者	日本獣医学会
収録物名	Journal of Veterinary Medical Science
巻(号)	78(7)
ページ	1117-1121
抄録	We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-7250
EISSN	1347-7439
NCID	AA10796138
関連情報	PMID 26983683
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.15-0701