Host-derived apolipoproteins play comparable roles with viral secretory proteins Erns and NS1 in the infectious particle formation of Flaviviridae

Fukuhara, Takasuke; Tamura, Tomokazu; Ono, Chikako; Shiokawa, Mai; Mori, Hiroyuki; Uemura, Kentaro; Yamamoto, Satomi; Kurihara, Takeshi; Okamoto, Toru; Suzuki, Ryosuke; Yoshii, Kentaro; Kurosu, Takeshi; Igarashi, Manabu; Aoki, Hiroshi; Sakoda, Yoshihiro; Matsuura, Yoshiharu

Host-derived apolipoproteins play comparable roles with viral secretory proteins Erns and NS1 in the infectious particle formation of Flaviviridae

Fukuhara, Takasuke; Tamura, Tomokazu; Ono, Chikako et al.

PLoS pathogens, 2017, 13(6), e1006475

Permalink : https://hdl.handle.net/2115/76370

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アクセス権	open access
URI	https://hdl.handle.net/2115/76370
タイトル	Host-derived apolipoproteins play comparable roles with viral secretory proteins Erns and NS1 in the infectious particle formation of Flaviviridae
著者	Fukuhara, Takasuke ; Tamura, Tomokazu ; Ono, Chikako ; Shiokawa, Mai ; Mori, Hiroyuki ; Uemura, Kentaro ; Yamamoto, Satomi ; Kurihara, Takeshi ; Okamoto, Toru ; Suzuki, Ryosuke ; Yoshii, Kentaro ; Kurosu, Takeshi ; Igarashi, Manabu ; Aoki, Hiroshi ; Sakoda, Yoshihiro ; Matsuura, Yoshiharu
e-Rad_Researcher 70598739 著者名 Fukuhara, Takasuke
著者名 Tamura, Tomokazu
e-Rad_Researcher 30626437 著者名 Ono, Chikako
著者名 Shiokawa, Mai
著者名 Mori, Hiroyuki
著者名 Uemura, Kentaro
著者名 Yamamoto, Satomi
著者名 Kurihara, Takeshi
e-Rad_Researcher 80628595 著者名 Okamoto, Toru
著者名 Suzuki, Ryosuke
e-Rad_Researcher 50421988 著者名 Yoshii, Kentaro
e-Rad_Researcher 70432432 著者名 Kurosu, Takeshi
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
e-Rad_Researcher 10440067 著者名 Aoki, Hiroshi
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
e-Rad_Researcher 50157252 著者名 Matsuura, Yoshiharu
言語	英語
発行日	2017-06-23
出版者	PLOS
収録物名	PLoS pathogens
巻(号)	13(6)
ページ	e1006475
抄録	Amphipathic α-helices of exchangeable apolipoproteins have shown to play crucial roles in the formation of infectious hepatitis C virus (HCV) particles through the interaction with viral particles. Among the Flaviviridae members, pestivirus and flavivirus possess a viral structural protein Erns or a non-structural protein 1 (NS1) as secretory glycoproteins, respectively, while Hepacivirus including HCV has no secretory glycoprotein. In case of pestivirus replication, the C-terminal long amphipathic α-helices of Erns are important for anchoring to viral membrane. Here we show that host-derived apolipoproteins play functional roles similar to those of virally encoded Erns and NS1 in the formation of infectious particles. We examined whether Erns and NS1 could compensate for the role of apolipoproteins in particle formation of HCV in apolipoprotein B (ApoB) and ApoE double-knockout Huh7 (BE-KO), and non-hepatic 293T cells. We found that exogenous expression of either Erns or NS1 rescued infectious particle formation of HCV in the BE-KO and 293T cells. In addition, expression of apolipoproteins or NS1 partially rescued the production of infectious pestivirus particles in cells upon electroporation with an Erns-deleted non-infectious RNA. As with exchangeable apolipoproteins, the C-terminal amphipathic α-helices of Erns play the functional roles in the formation of infectious HCV or pestivirus particles. These results strongly suggest that the host- and virus-derived secretory glycoproteins have overlapping roles in the viral life cycle of Flaviviridae, especially in the maturation of infectious particles, while Erns and NS1 also participate in replication complex formation and viral entry, respectively. Considering the abundant hepatic expression and liver-specific propagation of these apolipoproteins, HCV might have evolved to utilize them in the formation of infectious particles through deletion of a secretory viral glycoprotein gene.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1553-7366
EISSN	1553-7374
関連情報	PMID 28644867
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.ppat.1006475