Quinolone resistance-associated amino acid substitutions affect enzymatic activity of Mycobacterium leprae DNA gyrase

Yamaguchi, Tomoyuki; Yokoyama, Kazumasa; Nakajima, Chie; Suzuki, Yasuhiko

Quinolone resistance-associated amino acid substitutions affect enzymatic activity of Mycobacterium leprae DNA gyrase

Yamaguchi, Tomoyuki; Yokoyama, Kazumasa; Nakajima, Chie et al.

Bioscience, Biotechnology, and Biochemistry, 2017, 81(7), 1343-1347

Permalink : https://hdl.handle.net/2115/76493

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/76493
タイトル	Quinolone resistance-associated amino acid substitutions affect enzymatic activity of Mycobacterium leprae DNA gyrase
著者	Yamaguchi, Tomoyuki ; Yokoyama, Kazumasa ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名 Yamaguchi, Tomoyuki
著者名 Yokoyama, Kazumasa
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	DNA gyrase
	Mycobacterium leprae
	quinolone resistance
	amino acid substitution
	enzymatic activity
発行日	2017-04-18
出版者	Japan Society for Bioscience, Biotechnology, and Agrochemistry
収録物名	Bioscience, Biotechnology, and Biochemistry
巻(号)	81(7)
ページ	1343-1347
抄録	Quinolones are important antimicrobials for treatment of leprosy, a chronic infectious disease caused by Mycobacterium leprae. Although it is well known that mutations in DNA gyrase are responsible for quinolone resistance, the effect of those mutations on the enzymatic activity is yet to be studied in depth. Hence, we conducted in vitro assays to observe supercoiling reactions of wild type and mutated M. leprae DNA gyrases. DNA gyrase with amino acid substitution Ala91Val possessed the highest activity among the mutants. DNA gyrase with Gly89Cys showed the lowest level of activity despite being found in clinical strains, but it supercoiled DNA like the wild type does if applied at a sufficient concentration. In addition, patterns of time-dependent conversion from relaxed circular DNA into supercoiled DNA by DNA gyrases with clinically unreported Asp95Gly and Asp95Asn were observed to be distinct from those by the other DNA gyrases.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 18 Apr 2017, available online: http://www.tandfonline.com/10.1080/09168451.2017.1314757
PISSN	0916-8451
EISSN	1347-6947
NCID	AA10824164
関連情報	PMID 28417702
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1080/09168451.2017.1314757