An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A

Mazaafrianto, Donny Nugraha; Ishida, Akihiko; Maeki, Masatoshi; Tani, Hirofumi; Tokeshi, Manabu

An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A

Mazaafrianto, Donny Nugraha; Ishida, Akihiko; Maeki, Masatoshi et al.

Analytical sciences, 2019, 35(11), 1221-1226

Permalink : https://hdl.handle.net/2115/76500

このアイテムのアクセス数:610件（2026-10-10 02:40 集計）

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ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.2116/analsci.19P240			16

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/76500
タイトル	An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A
著者	Mazaafrianto, Donny Nugraha ; Ishida, Akihiko ; Maeki, Masatoshi ; Tani, Hirofumi ; Tokeshi, Manabu
著者名 Mazaafrianto, Donny Nugraha
e-Rad_Researcher 20312382 著者名 Ishida, Akihiko
著者名 Maeki, Masatoshi
e-Rad_Researcher 10271644 著者名 Tani, Hirofumi
e-Rad_Researcher 60311437 著者名 Tokeshi, Manabu
言語	英語
キーワード	Dithiol
	microfabricated device
	electrochemical detection
	aptamer
	methylene blue
	ochratoxin A
	signal-on
発行日	2019-11-10
出版者	Japan Society for Analytical Chemistry
出版者	日本分析化学会
収録物名	Analytical sciences
巻(号)	35(11)
ページ	1221-1226
抄録	In this study, we developed an electrochemical sensor for ochratoxin A (OTA) by using an aptamer having a dithiol-based anchor, which exhibited higher stability on a gold electrode than a monothiol-based aptamer because of its two anchors. The sensor was also based on a signal-on scheme that produces a signal current resulting from structure-switching of the aptamer upon interaction with OTA. For simple fabrication of this sensor, the non-covalent interaction of methylene blue with the aptamer was also employed as an electrochemical indicator. In this study, the performance of the sensor, including the dissociation constant of the aptamer-OTA complex, was characterized. The proposed sensor exhibited high reproducibility and enough sensitivity to detect the minimum amount of OTA required for the analysis of real food samples with a limit of detection of 113 pM.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0910-6340
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2116/analsci.19P240