Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos

LI, Jianye; MAEJI, Mana; BALBOULA, Ahmed Zaky; ABOELENAIN, Mansour; FUJII, Takashi; MORIYASU, Satoru; BAI, Hanako; KAWAHARA, Manabu; TAKAHASHI, Masashi

Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos

LI, Jianye; MAEJI, Mana; BALBOULA, Ahmed Zaky et al.

Journal of Reproduction and Development, 2019, 66(1), 9-17

Permalink : https://hdl.handle.net/2115/76590

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アクセス権	open access
URI	https://hdl.handle.net/2115/76590
タイトル	Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos
著者	LI, Jianye ; MAEJI, Mana ; BALBOULA, Ahmed Zaky ; ABOELENAIN, Mansour ; FUJII, Takashi ; MORIYASU, Satoru ; BAI, Hanako ; KAWAHARA, Manabu ; TAKAHASHI, Masashi
著者名 LI, Jianye
著者名 MAEJI, Mana
著者名 BALBOULA, Ahmed Zaky
著者名 ABOELENAIN, Mansour
著者名 FUJII, Takashi
著者名 MORIYASU, Satoru
e-Rad_Researcher 60775443 著者名 BAI, Hanako
e-Rad_Researcher 70468700 著者名 KAWAHARA, Manabu
e-Rad_Researcher 10343964 著者名 TAKAHASHI, Masashi
言語	英語
キーワード	Cathepsin B
	Cow
	Lysosome
	Oocytes
	Preimplantation embryos
発行日	2019
出版者	日本繁殖生物学会
収録物名	Journal of Reproduction and Development
巻(号)	66(1)
ページ	9-17
抄録	Lysosomal cathepsin, in particular cathepsin B (CTSB), plays an important role in implantation, pregnancy, and embryonic development. However, little is known about the mechanism related to the dynamic status of lysosomal cathepsins in bovine oocytes and preimplantation embryos. In the present study, we investigated the dynamics of gene expression, activity, and immunolocalization of CTSB, as well as the activities of lysosome, in bovine oocytes and preimplantation embryos. After gene expression analysis of several cathepsin-related genes, transcript levels of CTSB, CTSD and CTSZ were highest in MII oocytes followed by a significant decrease from the 8-cell embryo stage. Activity of CTSB showed a significant increase in 1-cell and morula stage embryos. Lysosomal activity was also significant higher in 1-cell and morula stages, which was consistent with CTSB activities. However, immunolocalization of CTSB did not show the similar pattern of CTSB and lysosomal activities. We also found significantly higher expression levels of CTSB transcript in the trophectoderm (TE) compared to inner cell mass (ICM), whereas activity and immunolocalization of CTSB showed an opposite pattern, i.e. significantly higher in ICM than TE. These patterns were confirmed by the same analysis using separated ICM and TE. Our results suggest that lysosomal CTSB has a pivotal role during embryonic development and differentiation, especially fertilization and the differentiation period.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license. https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0916-8818
EISSN	1348-4400
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1262/jrd.2019-115