Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes

LI, Jianye; BALBOULA, Ahmed Zaky; ABOELENAIN, Mansour; FUJII, Takashi; MORIYASU, Satoru; BAI, Hanako; KAWAHARA, Manabu; TAKAHASHI, Masashi

Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes

LI, Jianye; BALBOULA, Ahmed Zaky; ABOELENAIN, Mansour et al.

Journal of Reproduction and Development, 2019, 66(1), 83-91

Permalink : https://hdl.handle.net/2115/76591

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アクセス権	open access
URI	https://hdl.handle.net/2115/76591
タイトル	Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
著者	LI, Jianye ; BALBOULA, Ahmed Zaky ; ABOELENAIN, Mansour ; FUJII, Takashi ; MORIYASU, Satoru ; BAI, Hanako ; KAWAHARA, Manabu ; TAKAHASHI, Masashi
著者名 LI, Jianye
著者名 BALBOULA, Ahmed Zaky
著者名 ABOELENAIN, Mansour
著者名 FUJII, Takashi
著者名 MORIYASU, Satoru
e-Rad_Researcher 60775443 著者名 BAI, Hanako
e-Rad_Researcher 70468700 著者名 KAWAHARA, Manabu
e-Rad_Researcher 10343964 著者名 TAKAHASHI, Masashi
言語	英語
キーワード	Autophagy
	Cathepsin B
	Cow
	Developmental competence
	Oocyte quality
発行日	2019-12-25
出版者	日本繁殖生物学会
収録物名	Journal of Reproduction and Development
巻(号)	66(1)
ページ	83-91
抄録	The present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and poor germinal vesicle (GV) oocytes. Then E-64, a CTSB inhibitor, rapamycin (Rapa), an autophagy inducer, and combined administration was achieved during in vitro maturation (IVM) of poor quality COCs followed by detection of autophagy activity. In the next experiment, E-64, Rapa, and E64 + Rapa, were added during IVM to good and poor quality COCs followed by in vitro fertilization and culture for 8 days to investigate whether inhibition of CTSB and/or induction of autophagy improve embryonic development and quality. Autophagy activity was significantly lower in poor quality GV oocytes than in good quality ones. E-64, Rapa and E-64 + Rapa treatment during IVM significantly increased autophagy activity in poor quality oocytes. Addition of Rapa in good quality COCs did not increase the blastocyst rate, whereas E-64 increased the blastocyst rate and total cell number (TCN) with decreasing TUNEL-positive cells. In contrast, Rapa treatment in poor quality COCs significantly increased the blastocyst rate and TCN with decreasing TUNEL-positive cells. These results indicate oocyte quality has different responses to intracellular autophagy induction and CTSB activity control by potential autophagy and catabolic status, however, synergetic effect of autophagy induction and CTSB inhibition can increase developmental competence of both good and poor quality COCs, especially rescue effect in poor quality COCs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license. https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0916-8818
EISSN	1348-4400
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1262/jrd.2019-123