Heavy Water (D2O) Containing Preservation Solution Reduces Hepatic Cold Preservation and Reperfusion Injury in an Isolated Perfused Rat Liver (IPRL) Model

Shimada, Shingo; Fukai, Moto; Shibata, Kengo; Sakamoto, Sodai; Wakayama, Kenji; Ishikawa, Takahisa; Kawamura, Norio; Fujiyoshi, Masato; Shimamura, Tsuyoshi; Taketomi, Akinobu

Heavy Water (D2O) Containing Preservation Solution Reduces Hepatic Cold Preservation and Reperfusion Injury in an Isolated Perfused Rat Liver (IPRL) Model

Shimada, Shingo; Fukai, Moto; Shibata, Kengo et al.

Journal of clinical medicine, 2019, 8(11), 1818

Permalink : https://hdl.handle.net/2115/76618

このアイテムのアクセス数:561件（2026-08-24 16:42 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/76618
タイトル	Heavy Water (D2O) Containing Preservation Solution Reduces Hepatic Cold Preservation and Reperfusion Injury in an Isolated Perfused Rat Liver (IPRL) Model
著者	Shimada, Shingo ; Fukai, Moto ; Shibata, Kengo ; Sakamoto, Sodai ; Wakayama, Kenji ; Ishikawa, Takahisa ; Kawamura, Norio ; Fujiyoshi, Masato ; Shimamura, Tsuyoshi ; Taketomi, Akinobu
著者名 Shimada, Shingo
e-Rad_Researcher 60374344 著者名 Fukai, Moto
著者名 Shibata, Kengo
著者名 Sakamoto, Sodai
e-Rad_Researcher 50646544 著者名 Wakayama, Kenji
著者名 Ishikawa, Takahisa
e-Rad_Researcher 20746953 著者名 Kawamura, Norio
著者名 Fujiyoshi, Masato
e-Rad_Researcher 00333617 著者名 Shimamura, Tsuyoshi
e-Rad_Researcher 70363364 著者名 Taketomi, Akinobu
言語	英語
キーワード	heavy water
	D2O
	liver
	cold preservation
	mitochondria
	cytoskeleton
発行日	2019-11
出版者	MDPI
収録物名	Journal of clinical medicine
巻(号)	8(11)
ページ	1818
抄録	Background: Heavy water (D2O) has many biological effects due to the isotope effect of deuterium. We previously reported the efficacy of D2O containing solution (Dsol) in the cold preservation of rat hearts. Here, we evaluated whether Dsol reduced hepatic cold preservation and reperfusion injury. Methods: Rat livers were subjected to 48-hour cold storage in University of Wisconsin (UW) solution or Dsol, and subsequently reperfused on an isolated perfused rat liver. Graft function, injury, perfusion kinetics, oxidative stress, and cytoskeletal integrity were assessed. Results: In the UW group, severe ischemia and reperfusion injury (IRI) was shown by histopathology, higher liver enzymes leakage, portal resistance, and apoptotic index, oxygen consumption, less bile production, energy charge, and reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio (versus control). The Dsol group showed that these injuries were significantly ameliorated (versus the UW group). Furthermore, cytoskeletal derangement was progressed in the UW group, as shown by less degradation of alpha-Fodrin and by the inactivation of the actin depolymerization pathway, whereas these changes were significantly suppressed in the Dsol group. Conclusion: Dsol reduced hepatic IRI after extended cold preservation and subsequent reperfusion. The protection was primarily due to the maintenance of mitochondrial function, cytoskeletal integrity, leading to limiting oxidative stress, apoptosis, and necrosis pathways.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/jcm8111818