Analysis of the effects of polyunsaturated fatty acids on transporter expressions using a PCR array: Induction of xCT/SLC7A11 in human placental BeWo cells.

Ono, Kanako; Furugen, Ayako; Kurosawa, Yuko; Jinno, Naoko; Narumi, Katsuya; Kobayashi, Masaki; Iseki, Ken

Analysis of the effects of polyunsaturated fatty acids on transporter expressions using a PCR array: Induction of xCT/SLC7A11 in human placental BeWo cells.

Ono, Kanako; Furugen, Ayako; Kurosawa, Yuko et al.

Placenta, 2019, 75, 34-41

Permalink : https://hdl.handle.net/2115/76644

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アクセス権	open access
URI	https://hdl.handle.net/2115/76644
タイトル	Analysis of the effects of polyunsaturated fatty acids on transporter expressions using a PCR array: Induction of xCT/SLC7A11 in human placental BeWo cells.
著者	Ono, Kanako ; Furugen, Ayako ; Kurosawa, Yuko ; Jinno, Naoko ; Narumi, Katsuya ; Kobayashi, Masaki ; Iseki, Ken
著者名 Ono, Kanako
e-Rad_Researcher 90767261 著者名 Furugen, Ayako
著者名 Kurosawa, Yuko
著者名 Jinno, Naoko
e-Rad_Researcher 90746752 著者名 Narumi, Katsuya
e-Rad_Researcher 70431319 著者名 Kobayashi, Masaki
e-Rad_Researcher 40203062 著者名 Iseki, Ken
言語	英語
キーワード	Arachidonic acid (AA)
	Docosahexaenoic acid (DHA)
	Eicosapentaenoic acid (EPA)
	Placenta; Transporter
	xCT/SLC7A11
発行日	2019-01
収録物名	Placenta
巻(号)	75
ページ	34-41
抄録	OBJECTIVE: Polyunsaturated fatty acids (PUFAs), including arachidonic acid (AA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are essential for adequate fetal growth. The aim of the present study was to elucidate the effects of PUFAs on the expression and function of placental transporters, which play important roles in placental functions including the supply of nutrients to the fetus, excretion of metabolites, and protection of the fetus from xenobiotics. METHODS: Human placental choriocarcinoma BeWo cells were used as a trophoblast model. PUFA-induced alteration in the gene expression of 84 transporters was investigated by a commercially available PCR array. Protein levels and the activity of transporters were assessed by western blotting and uptake experiments, respectively. The placental expression of the transporters was analyzed using pregnant Wistar rats. RESULTS: PUFAs (AA, EPA, and DHA) increased cystine/glutamate transporter xCT/SLC7A11, which mediates the cellular uptake of cystine coupled with the efflux of glutamate in human placental choriocarcinoma BeWo cells. These PUFAs also increased [14C]-cystine uptake in BeWo cells. PUFA-induced xCT/SLC7A11 mRNA expression was not blocked by nuclear factor-erythroid 2-related factor-2 (NRF2) knockdown. Reverse transcription (RT)-PCR analysis indicated that xCT/Slc7a11 mRNA was detected in rat placenta and the expression level at gestational day (GD) 12 was higher than that at GD 20. CONCLUSION: These results indicate that PUFAs promoted cystine uptake in placental cells by inducing xCT/SLC7A11 expression and NRF2 did not contribute to upregulation of xCT/SLC7A11 by PUFAs. Furthermore, xCT expression in rat placenta may change during pregnancy.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1532-3102
関連情報	PMID 30712664
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.placenta.2018.11.010