The pH-dependent conformational change of eukaryotic translation initiation factor 5: Insights into partner-binding manner

Ye, Yuxin; Chen, Meirong; Kato, Koji; Yao, Min

The pH-dependent conformational change of eukaryotic translation initiation factor 5: Insights into partner-binding manner

Ye, Yuxin; Chen, Meirong; Kato, Koji et al.

Biochemical and biophysical research communications, 2019, 519(1), 186-191

Permalink : https://hdl.handle.net/2115/76740

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アクセス権	open access
URI	https://hdl.handle.net/2115/76740
タイトル	The pH-dependent conformational change of eukaryotic translation initiation factor 5: Insights into partner-binding manner
著者	Ye, Yuxin ; Chen, Meirong ; Kato, Koji ; Yao, Min
著者名 Ye, Yuxin
著者名 Chen, Meirong
著者名 Kato, Koji
e-Rad_Researcher 40311518 著者名 Yao, Min
言語	英語
キーワード	elF5
	AA-box
	eIF2β
	K-box
	Structure
発行日	2019-10-29
出版者	Elsevier
収録物名	Biochemical and biophysical research communications
巻(号)	519(1)
ページ	186-191
抄録	In the process of eukaryotic translation, the formation of preinitiation complex 43S, which consists of a 40S subunit, the eIF2-GTP-Met-tRNAi(Met) ternary complex, eIF3, eIF1, eIF1A, and eIF5, is essential for translational quality control. Of those factors, eIF5 promotes the hydrolysis of eIF2-bound GTP to release eIF2-GDP in the complex for the recycling of eIF2. eIF5 appears to bind to the beta subunit of eIF2 (eIF2 beta) via an interaction between aromatic/acidic residue-rich regions (AA-boxes) in the C-terminal domain of eIF5 (eIF5CTD) and three lysine clusters (K-boxes) in the N-terminal domain of eIF2 beta (eIF2 beta NTD). However, the details of this interaction are unclear, due to the lack of a structure for the eIF5-eIF2 beta complex, and the unavailability of an intact structure of eIF5, in which the AA-boxes are always disordered, with high flexibility. In this study, we solved two crystal structures of eIF5CTD from Candida albicans, which for the first time showed the AA-box2 of eIF5 presenting as an ordered helical structure. The structures exhibited different arrangements of AA-box2 under different pH values, which may reflect the dynamic nature of the interactions of eIF5CTD, and eIF2 beta NTD in the preinitiation complex. (C) 2019 The Authors. Published by Elsevier Inc.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0006-291X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.bbrc.2019.08.128