Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts

Yamamoto, Kei; Otomo, Kohei; Nemoto, Tomomi; Ishihara, Seiichiro; Haga, Hisashi; Nagasaki, Akira; Murakami, Yota; Takahashi, Masayuki

Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts

Yamamoto, Kei; Otomo, Kohei; Nemoto, Tomomi et al.

Experimental cell research, 2019, 376(1), 67-76

Permalink : https://hdl.handle.net/2115/76831

このアイテムのアクセス数:673件（2026-08-17 23:39 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/76831
タイトル	Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts
著者	Yamamoto, Kei ; Otomo, Kohei ; Nemoto, Tomomi ; Ishihara, Seiichiro ; Haga, Hisashi ; Nagasaki, Akira ; Murakami, Yota ; Takahashi, Masayuki
著者名 Yamamoto, Kei
e-Rad_Researcher 40547204 著者名 Otomo, Kohei
e-Rad_Researcher 50291084 著者名 Nemoto, Tomomi
e-Rad_Researcher 10719933 著者名 Ishihara, Seiichiro
e-Rad_Researcher 00292045 著者名 Haga, Hisashi
e-Rad_Researcher 30392640 著者名 Nagasaki, Akira
e-Rad_Researcher 20260622 著者名 Murakami, Yota
e-Rad_Researcher 50241295 著者名 Takahashi, Masayuki
言語	英語
キーワード	Nonmuscle myosin IIA
	Nonmuscle myosin IlB
	Contractile ring
	Cytokinesis
	Three-dimensional imaging
	Cortical stiffness
発行日	2019-03-01
出版者	Elsevier
収録物名	Experimental cell research
巻(号)	376(1)
ページ	67-76
抄録	Nonmuscle myosin II (NMII) plays an important role in cytokinesis by constricting a contractile ring. However, it is poorly understood how NMII isoforms contribute to cytokinesis in mammalian cells. Here, we investigated the roles of the two major NMII isoforms, NMIIA and NMIIB, in cytokinesis using a WI-38 VA13 cell line (human immortalized fibroblast). In this cell line, NMIIB tended to localize to the contractile ring more than NMIIA. The expression level of NMIIA affected the localization of NMIIB. Most NMIIB accumulated at the cleavage furrow in NMIIA-knockout (KO) cells, and most NMIIA was displaced from this location in exogenous NMIIB-expressing cells, indicating that NMIIB preferentially localizes to the contractile ring. Specific KO of each isoform elicited opposite effects. The rate of furrow ingression was decreased and increased in NMIIA-KO and NMIIB-KO cells, respectively. Meanwhile, the length of NMII-filament stacks in the contractile ring was increased and decreased in NMIIA-KO and NMIIB-KO cells, respectively. Moreover, NMIIA helped to maintain cortical stiffness during cytokinesis. These findings suggest that appropriate ratio of NMIIA and NMIIB in the contractile ring is important for proper cytokinesis in specific cell types. In addition, two-photon excitation spinning-disk confocal microscopy enabled us to image constriction of the contractile ring in live cells in a three-dimensional manner.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0014-4827
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.yexcr.2019.01.020