Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species

Kawai, Yusuke K.; Shinya, So; Ikenaka, Yoshinori; Saengtienchai, Aksorn; Kondo, Takamitsu; Darvvish, Wageh Sobhy; Nakayama, Shota M. M.; Mizukawa, Hazuki; Ishizuka, Mayumi

Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species

Kawai, Yusuke K.; Shinya, So; Ikenaka, Yoshinori et al.

Comparative biochemistry and physiology Part C: Toxicology & pharmacology, 2019, 217, 5-14

Permalink : https://hdl.handle.net/2115/76849

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アクセス権	open access
URI	https://hdl.handle.net/2115/76849
タイトル	Characterization of function and genetic feature of UDP-glucuronosyltransferase in avian species
著者	Kawai, Yusuke K. ; Shinya, So ; Ikenaka, Yoshinori ; Saengtienchai, Aksorn ; Kondo, Takamitsu ; Darvvish, Wageh Sobhy ; Nakayama, Shota M. M. ; Mizukawa, Hazuki ; Ishizuka, Mayumi
著者名 Kawai, Yusuke K.
著者名 Shinya, So
著者名 Ikenaka, Yoshinori
著者名 Saengtienchai, Aksorn
著者名 Kondo, Takamitsu
著者名 Darvvish, Wageh Sobhy
著者名 Nakayama, Shota M. M.
e-Rad_Researcher 60612661 著者名 Mizukawa, Hazuki
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	UGTs
	Xenobiotic
	Bird
	Wildlife
	Toxicology
発行日	2019-03
出版者	Elsevier
収録物名	Comparative biochemistry and physiology Part C: Toxicology & pharmacology
巻(号)	217
ページ	5-14
抄録	Birds are exposed to many xenobiotics during their lifetime. For accurate prediction of xenobiotic-induced toxic effects on avian species, it is necessary to understand metabolic capacities in a comprehensive range of bird species. However, there is a lack of information about avian xenobiotic metabolizing enzymes (XMEs), particularly in wild birds. Uridine diphosphate glucuronosyltransferase (UGT) is an XME that plays an important role in phase II metabolism in the livers of mammals and birds. This study was performed to determine the characteristics of UGT1E isoform in avian species, those are related to mammals UGT 1A. To understand the characteristics of avian UGT1E isoforms, in vitro metabolic activity and genetic characteristics were investigated. Furthermore, mRNA expression levels of all chicken UGT1E isoforms were measured. On in vitro enzymatic analysis, the white-tailed eagle, great horned owl, and Humboldt penguin showed lower UGT-dependent activity than domestic birds. In synteny analysis, carnivorous birds were shown to have fewer UGT1E isoforms than herbivorous and omnivorous birds, which may explain why they have lower in vitro UGT activity. These observations suggested that raptors and seabirds, in which UGT activity is low, may be at high risk if exposed to elevated levels of xenobiotics in the environment. Phylogenetic analysis suggested that avian UGT1Es have evolved independently from mammalian UGT1As. We identified the important UGT isoforms, such as UGT1E13, and suspected their substrate specificities in avian xenobiotic metabolism by phylogenetic and quantitative realtime PCR analysis. This is the first report regarding the genetic characteristics and interspecies differences of UGT1Es in avian species.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	1532-0456
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.cbpc.2018.11.001