Structure of HIV-2 Nef Reveals Features Distinct from HIV-1 Involved in Immune Regulation

Hirao, Kengo; Andrews, Sophie; Kuroki, Kimiko; Kusaka, Hiroki; Tadokoro, Takashi; Kita, Shunsuke; Ose, Toyoyuki; Rowland-Jones, Sarah L.; Maenaka, Katsumi

Structure of HIV-2 Nef Reveals Features Distinct from HIV-1 Involved in Immune Regulation

Hirao, Kengo; Andrews, Sophie; Kuroki, Kimiko et al.

iScience, 2020, 23(1), 100758

Permalink : https://hdl.handle.net/2115/76924

このアイテムのアクセス数:727件（2026-08-27 08:47 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/76924
タイトル	Structure of HIV-2 Nef Reveals Features Distinct from HIV-1 Involved in Immune Regulation
著者	Hirao, Kengo ; Andrews, Sophie ; Kuroki, Kimiko ; Kusaka, Hiroki ; Tadokoro, Takashi ; Kita, Shunsuke ; Ose, Toyoyuki ; Rowland-Jones, Sarah L. ; Maenaka, Katsumi
著者名 Hirao, Kengo
著者名 Andrews, Sophie
e-Rad_Researcher 90553313 著者名 Kuroki, Kimiko
著者名 Kusaka, Hiroki
e-Rad_Researcher 10762396 著者名 Tadokoro, Takashi
著者名 Kita, Shunsuke
著者名 Ose, Toyoyuki
著者名 Rowland-Jones, Sarah L.
e-Rad_Researcher 10322752 著者名 Maenaka, Katsumi
言語	英語
発行日	2020-01-24
出版者	Cell Press
収録物名	iScience
巻(号)	23(1)
ページ	100758
抄録	The human immunodeficiency virus (HIV) accessory protein Nef plays a major role in establishing and maintaining infection, particularly through immune evasion. Many HIV-2-infected people experience long-term viral control and survival, resembling HIV-1 elite control. HIV-2 Nef has overlapping but also distinct functions from HIV-1 Nef. Here we report the crystal structure of HIV-2 Nef core. The di-leucine sorting motif forms a helix bound to neighboring molecules, and moreover, isothermal titration calorimetry demonstrated that the CD3 endocytosis motif can directly bind to HIV-2 Nef, ensuring AP-2-mediated endocytosis for CD3. The highly conserved C-terminal region forms a alpha-helix, absent from HIV-1. We further determined the structure of simian immunodeficiency virus (SIV) Nef harboring this region, demonstrating similar C-terminal a-helix, which may contribute to AP-1 binding for MHC-I downregulation. These results provide insights into the distinct pathogenesis of HIV-2 infection.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.isci.2019.100758