First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia

Hala, Sharif; Antony, Chakkiath Paul; Alshehri, Mohammed; Althaqafi, O. Abdulhakeem; Alsaedi, Asim; Mufti, Areej; Kaaki, Mai; Alhaj-Hussein, T. Baraa; Zowawi, M. Hosam; Al-Amri, Abdulfattah; Pain, Arnab

First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia

Hala, Sharif; Antony, Chakkiath Paul; Alshehri, Mohammed et al.

Antimicrobial resistance and infection control, 2019, 8(1), 203

Permalink : https://hdl.handle.net/2115/76928

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アクセス権	open access
URI	https://hdl.handle.net/2115/76928
タイトル	First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia
著者	Hala, Sharif ; Antony, Chakkiath Paul ; Alshehri, Mohammed ; Althaqafi, O. Abdulhakeem ; Alsaedi, Asim ; Mufti, Areej ; Kaaki, Mai ; Alhaj-Hussein, T. Baraa ; Zowawi, M. Hosam ; Al-Amri, Abdulfattah ; Pain, Arnab
著者名 Hala, Sharif
著者名 Antony, Chakkiath Paul
著者名 Alshehri, Mohammed
著者名 Althaqafi, O. Abdulhakeem
著者名 Alsaedi, Asim
著者名 Mufti, Areej
著者名 Kaaki, Mai
著者名 Alhaj-Hussein, T. Baraa
著者名 Zowawi, M. Hosam
著者名 Al-Amri, Abdulfattah
著者名 Pain, Arnab
言語	英語
キーワード	MDR
	Klebsiella quasipneumoniae
	Carbapenemases
	KPC-2
	bla(KPC-2)
	Tn3
発行日	2019-12-19
出版者	BioMed Central
収録物名	Antimicrobial resistance and infection control
巻(号)	8(1)
ページ	203
抄録	Background Nosocomial infections caused by multi-drug resistant Enterobacteriaceae are a global public health threat that ought to be promptly identified, reported, and addressed accurately. Many carbapenem-resistant Enterobacteriaceae-associated genes have been identified in Saudi Arabia but not the endemic Klebsiella pneumoniae carbapenemases (KPCs), which are encoded by bla(KPC-type) genes. KPCs are known for their exceptional spreading potential. Methods We collected n = 286 multi-drug resistant (MDR) Klebsiella spp. isolates as part of screening for resistant patterns from a tertiary hospital in Saudi Arabia between 2014 and 2018. Antimicrobial susceptibility testing was carried out using both VITEK II and the broth microdilution of all collected isolates. Detection of resistance-conferring genes was carried out using Illumina whole-genome shotgun sequencing and PacBio SMRT sequencing protocols. Results A Carbapenem-resistant Enterobacteriaceae (CRE) Klebsiella quasipneumoniae subsp. similipneumoniae strain was identified as a novel ST-3510 carrying a bla(KPC-2) carbapenemase encoding gene. The isolate, designated as NGKPC-421, was obtained from shotgun Whole Genome Sequencing (WGS) surveillance of 286 MDR Klebsiella spp. clinical isolates. The NGKPC-421 isolate was collected from a septic patient in late 2017 and was initially misidentified as K. pneumoniae. The sequencing and assembly of the NGKPC-421 genome resulted in the identification of a putative similar to 39.4 kb IncX6 plasmid harboring a bla(KPC-2) gene, flanked by transposable elements (ISKpn6-bla(KPC-2)-ISKpn27). Conclusion This is the first identification of a KPC-2-producing CRE in the Gulf region. The impact on this finding is of major concern to the public health in Saudi Arabia, considering that it is the religious epicenter with a continuous mass influx of pilgrims from across the world. Our study strongly highlights the importance of implementing rapid sequencing-based technologies in clinical microbiology for precise taxonomic classification and monitoring of antimicrobial resistance patterns.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
EISSN	2047-2994
関連情報	PMID 31890159
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s13756-019-0653-9