open access

https://doi.org/10.14943/doctoral.k13487

https://hdl.handle.net/2115/77150

surface pre-reacted glassionomer（S-PRG）ナノフィラーによるヒト象牙質表面の抗菌コーティング

英語

Streptococcus mutans

2019-03-25

Hokkaido University

Surface pre-reacted glass ionomer (S-PRG) filler shows tooth remineralization and antibacterial effects via ion-releasing. In this study, we fabricated the nano- and micro-sized S-PRG fillers and assessed the adhesive properties of S-PRG fillers to the human dentin surface. In addition, the antibacterial effects of dentin coated with S-PRG fillers were examined. The stock of S-PRG filler (average particle size; 5 μm) was fractionated to obtain the two types of dispersion; nano and micro scale particle types, and then labeled with nano- and micro-S-PRG, respectively. S-PRG fillers were characterized by scanning electron microscope (SEM), energy dispersive X-ray spectrometry (EDX), ion-releasing test and provided to antibacterial and cytotoxic assessments. Subsequently, we assessed the adhesion test of stock, nano- and micro-S-PRG to human dentin blocks. In addition, antibacterial effects of nano-S-PRG coated dentin was examined using Streptococcus mutans and Actinomyces naeslundii. The results showed that the nano- and micro-S-PRG showed including of elements of F, Na, Al, Si and Sr, ion releasing ability comparable with the stock, antimicrobial activity and low cytotoxicity. SEM and EDX examinations revealed that S-PRG fillers uniformly covered the dentin surface after application of filler to dentin. After ultrasonically cleaning, nano-S-PRG predominantly remained on the dentin surface compared to stock and micro-S-PRG, suggesting that the nano-S-PRG could adhered to dentin. Nano-S-PRG treated dentin showed the sterilization effect and bacterial growth inhibition. In conclusion, nano-S-PRG exhibited great adhesive and antimicrobial properties to human dentin. S-PRG application to tooth would be beneficial for dental antibacterial treatment.

2019-03-25

甲第13487号

博士(歯学)

(主査) 特任教授 柴田 健一郎, 教授 吉田 靖弘, 准教授 菅谷 勉

歯学研究科(口腔医学専攻)

博士論文

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