Effect of thermal control of dry fomites on regulating the survival of human pathogenic bacteria responsible for nosocomial infections

Shimoda, Tomoko; Okubo, Torahiko; Enoeda, Yoshiki; Yano, Rika; Nakamura, Shinji; Thapa, Jeewan; Yamaguchi, Hiroyuki

Effect of thermal control of dry fomites on regulating the survival of human pathogenic bacteria responsible for nosocomial infections

Shimoda, Tomoko; Okubo, Torahiko; Enoeda, Yoshiki et al.

PLoS ONE, 2019, 14(12), e0226952

Permalink : https://hdl.handle.net/2115/77494

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アクセス権	open access
URI	https://hdl.handle.net/2115/77494
タイトル	Effect of thermal control of dry fomites on regulating the survival of human pathogenic bacteria responsible for nosocomial infections
著者	Shimoda, Tomoko ; Okubo, Torahiko ; Enoeda, Yoshiki ; Yano, Rika ; Nakamura, Shinji ; Thapa, Jeewan ; Yamaguchi, Hiroyuki
e-Rad_Researcher 60576180 著者名 Shimoda, Tomoko
e-Rad_Researcher 90762196 著者名 Okubo, Torahiko
著者名 Enoeda, Yoshiki
e-Rad_Researcher 50250519 著者名 Yano, Rika
e-Rad_Researcher 40207882 著者名 Nakamura, Shinji
著者名 Thapa, Jeewan
e-Rad_Researcher 40221650 著者名 Yamaguchi, Hiroyuki
言語	英語
発行日	2019-12-27
出版者	PLOS
収録物名	PLoS ONE
巻(号)	14(12)
ページ	e0226952
抄録	We monitored the survival of human pathogenic bacteria [Escherichia coli (ATCC), extended-spectrum beta-lactamase-producing E. coli (Clinical isolate), New Delhi metallo-beta-lactamase-producing E. coli (clinical isolate), Staphylococcus aureus (ATCC)] on dry materials (vinyl chloride, aluminum, plastic, stainless steel) at distinct temperatures (room temperature or 15 degrees C-37 degrees C). These bacteria favored a lower temperature for their prolonged survival on the dry fomites, regardless of the material type. Interestingly, when mixed with S. aureus, E. coli survived for a longer time at a lower temperature. Cardiolipin, which can promote the survival of S. aureus in harsh environments, had no effect on maintaining the survival of E. coli. Although the trends remained unchanged, adjusting the humidity from 40% to 60% affected the survival of bacteria on dry surfaces. Scanning electron microscopic analysis revealed no morphological differences in these bacteria immediately before or after one day of dry conditions. In addition, ATP assessment, a method used to visualize high-touch surfaces in hospitals, was not effective at monitoring bacterial dynamics. A specialized handrail device fitted with a heater, which was maintained at normal human body core temperature, successfully prohibited the prolonged survival of bacteria [Enterococcus faecalis (ATCC), E. coli (ATCC), Pseudomonas aeruginosa (ATCC), S. aureus (ATCC), Acinetobacter baumannii (clinical isolate), and Serratia marcescens (clinical isolate)], with the exception of spore-forming Bacillus subtilis (from our laboratory collection) and the yeast like fungus Candida albicans (from our laboratory collection)] on dry surfaces. Taken together, we concluded that the tested bacteria favor lower temperatures for their survival in dry environments. Therefore, the thermal control of dry fomites has the potential to control bacterial survival on high-touch surfaces in hospitals.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1932-6203
関連情報	PMID 31881059
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0226952