Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique

Miyazaki, Takuji; Hanamatsu, Hisatoshi; Xu, Liang; Onodera, Tomohiro; Furukawa, Jun-ichi; Homan, Kentaro; Baba, Rikiya; Kawasaki, Toshisuke; Iwasaki, Norimasa

Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique

Miyazaki, Takuji; Hanamatsu, Hisatoshi; Xu, Liang et al.

International Journal of Molecular Sciences, 2020, 21(1), 231

Permalink : https://hdl.handle.net/2115/77500

このアイテムのアクセス数:592件（2026-08-16 20:06 集計）

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https://doi.org/10.3390/ijms21010231			14

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/77500
タイトル	Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique
著者	Miyazaki, Takuji ; Hanamatsu, Hisatoshi ; Xu, Liang ; Onodera, Tomohiro ; Furukawa, Jun-ichi ; Homan, Kentaro ; Baba, Rikiya ; Kawasaki, Toshisuke ; Iwasaki, Norimasa
著者名 Miyazaki, Takuji
e-Rad_Researcher 70734185 著者名 Hanamatsu, Hisatoshi
著者名 Xu, Liang
e-Rad_Researcher 70547174 著者名 Onodera, Tomohiro
e-Rad_Researcher 30374193 著者名 Furukawa, Jun-ichi
e-Rad_Researcher 40823331 著者名 Homan, Kentaro
著者名 Baba, Rikiya
著者名 Kawasaki, Toshisuke
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
言語	英語
キーワード	cartilage damage
	osteoarthritis
	iPSCs
	chondrocytes
	GSL-glycome analysis
	aminolysis-SALSA
	tumorigenicity
	glycoconjugates
発行日	2020-01-01
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	21(1)
ページ	231
抄録	Cartilage damage may eventually lead to osteoarthritis because it is difficult to repair. Human-induced pluripotent stem cell (iPSC)-derived chondrocytes may potentially be used to treat cartilage damage, but the tumorigenicity of iPSCs is a major concern for their application in regenerative medicine. Many glycoconjugates serve as stem cell markers, and glycosphingolipids (GSLs) including H type 1 antigen (Fuc alpha 1-2Gal beta 1-3GlcNAc) have been expressed on the surface of iPSCs. The purpose of the present study was to investigate whether GSL-glycome analysis is useful for quality control of residual iPSCs in chondrocytes. We performed GSL-glycome analysis of undifferentiated iPSCs in chondrocytes by combining glycoblotting and aminolysis-sialic acid linkage-specific alkylamidation (SALSA) method, enabling the detection of small quantities of iPSC-specific GSL-glycans from 5 x 10(4) cells. Furthermore, we estimated the residual amount of iPSCs using R-17F antibody, which possesses cytotoxic activity toward iPSCs that is dependent on the Lacto-N-fucopentaose I (LNFP I) of GSL. Moreover, we could detect a small number of LNFP I during mesenchymal stem cells (MSCs) differentiation from iPSCs. This is the first demonstration that GSL-glycome analysis is useful for detecting undifferentiated iPSCs, and can thereby support safe regenerative medicine.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms21010231