Detection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV

Yamamoto, Keikokw; Ohnishi, Shunsuke; Mizushima, Takeshi; Kodaira, Junichi; Ono, Masayoshi; Hatanaka, Yutaka; Hatanaka, Kanako C.; Kuriki, Yugo; Kamiya, Mako; Ehira, Nobuyuki; Shinada, Keisuke; Takahashi, Hiroaki; Shimizu, Yuichi; Urano, Yasuteru; Sakamoto, Naoya

Detection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV

Yamamoto, Keikokw; Ohnishi, Shunsuke; Mizushima, Takeshi et al.

BMC cancer, 2020, 20(1), 64

Permalink : https://hdl.handle.net/2115/77824

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アクセス権	open access
URI	https://hdl.handle.net/2115/77824
タイトル	Detection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV
著者	Yamamoto, Keikokw ; Ohnishi, Shunsuke ; Mizushima, Takeshi ; Kodaira, Junichi ; Ono, Masayoshi ; Hatanaka, Yutaka ; Hatanaka, Kanako C. ; Kuriki, Yugo ; Kamiya, Mako ; Ehira, Nobuyuki ; Shinada, Keisuke ; Takahashi, Hiroaki ; Shimizu, Yuichi ; Urano, Yasuteru ; Sakamoto, Naoya
著者名 Yamamoto, Keikokw
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
著者名 Mizushima, Takeshi
著者名 Kodaira, Junichi
著者名 Ono, Masayoshi
e-Rad_Researcher 30589924 著者名 Hatanaka, Yutaka
著者名 Hatanaka, Kanako C.
著者名 Kuriki, Yugo
e-Rad_Researcher 90596462 著者名 Kamiya, Mako
著者名 Ehira, Nobuyuki
著者名 Shinada, Keisuke
著者名 Takahashi, Hiroaki
e-Rad_Researcher 90333608 著者名 Shimizu, Yuichi
著者名 Urano, Yasuteru
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	Fluorescence imaging
	Dipeptidyl peptidase-IV
	Adenocarcinoma of the esophagogastric junction
発行日	2020-01-28
出版者	BioMed Central
収録物名	BMC cancer
巻(号)	20(1)
ページ	64
抄録	Background It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7, 85.7, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s12885-020-6537-9