open access

https://hdl.handle.net/2115/78160

Elucidation of biosynthetic pathway of a plant hormone abscisic acid in phytopathogenic fungi

英語

phytopathogenic fungi

2020-05-21

Taylor & Francis

Bioscience biotechnology and biochemistry

83(9)

1642-1649

Abscisic acid (ABA) is one of the plant hormones that regulates physiological functions in various organisms, including plants, sponges, and humans. The biosynthetic machinery in plants is firmly established, while that in fungi is still unclear. Here, we elucidated the functions of the four biosynthetic genes, bcABA1-bcABA4, found in Botrytis cinerea by performing biotransformation experiments and in vitro enzymatic reactions with putative biosynthetic intermediates. The first-committed step is the cyclization of farnesyl diphosphate to give alpha-ionylideneethane catalyzed by a novel sesquiterpene synthase, BcABA3, which exhibits low amino acid sequence identities with sesquiterpene synthases. Subsequently, two cytochrome P450s, BcABA1 and BcABA2, mediate oxidative modifications of the cyclized product to afford 1MODIFIER LETTER PRIME,4MODIFIER LETTER PRIME-trans-dihydroxy-alpha-ionylideneacetic acid, which undergoes alcohol oxidation to furnish ABA. Our results demonstrated that production of ABA does not depend on the nucleotide sequence of bcABA genes. The present study set the stage to investigate the role of ABA in infections.

学術雑誌論文

Accepted Manuscript

This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 21 May 2019, available online: http://www.tandfonline.com/10.1080/09168451.2019.1618700.

0916-8451

AA10824164