Pharmaceutical immunoglobulins reduce neutrophil extracellular trap formation and ameliorate the development of MPO-ANCA-associated vasculitis

Uozumi, Ryo; Iguchi, Risa; Masuda, Sakiko; Nishibata, Yuka; Nakazawa, Daigo; Tomaru, Utano; Ishizu, Akihiro

Pharmaceutical immunoglobulins reduce neutrophil extracellular trap formation and ameliorate the development of MPO-ANCA-associated vasculitis

Uozumi, Ryo; Iguchi, Risa; Masuda, Sakiko et al.

Modern Rheumatology, 2019, 30(3), 544-550

Permalink : https://hdl.handle.net/2115/78182

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アクセス権	open access
URI	https://hdl.handle.net/2115/78182
タイトル	Pharmaceutical immunoglobulins reduce neutrophil extracellular trap formation and ameliorate the development of MPO-ANCA-associated vasculitis
著者	Uozumi, Ryo ; Iguchi, Risa ; Masuda, Sakiko ; Nishibata, Yuka ; Nakazawa, Daigo ; Tomaru, Utano ; Ishizu, Akihiro
著者名 Uozumi, Ryo
著者名 Iguchi, Risa
著者名 Masuda, Sakiko
著者名 Nishibata, Yuka
著者名 Nakazawa, Daigo
e-Rad_Researcher 20360901 著者名 Tomaru, Utano
e-Rad_Researcher 60321957 著者名 Ishizu, Akihiro
言語	英語
キーワード	ANCA-associated vasculitis
	Animal model
	Anti-neutrophil cytoplasmic antibody (ANCA)
	Intravenous immunoglobulin (IVIG)
	Neutrophil extracellular traps (NETs)
発行日	2019-04-12
出版者	Taylor & Francis
収録物名	Modern Rheumatology
巻(号)	30(3)
ページ	544-550
抄録	Objectives: Intravenous immunoglobulin (IVIG) therapy is effective against some autoimmune diseases. Although its efficacy on peripheral neuropathy due to eosinophilic granulomatosis with polyangiitis-one of myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)-associated vasculitis (MPO-AAV)-has been established, that on other MPO-AAV remains undetermined. We examined the effects of pharmaceutical immunoglobulins on the formation of neutrophil extracellular traps (NETs) related to MPO-ANCA production and the development of MPO-AAV. Methods: Peripheral blood neutrophils from healthy volunteers were pretreated with 5 mg/ml human sulfo-immunoglobulins (IVIG-S) and then exposed to 100 nM phorbol myristate acetate (PMA). Thereafter, neutrophils were stained with SYTOX Green and then subjected to flow cytometry. Next, Wistar-Kyoto rats were given oral administration of 10 mg/kg/day propylthiouracil for 28 days and intraperitoneal (i.p.) injection of 1 µg PMA on days 0 and 7. These rats were divided into two groups: Group 1 with i.p. injection of 400 mg/kg IVIG-S on days 8-12 and Group 2 with i.p. injection of vehicle similarly. ANCA titers were chronologically determined by indirect immunofluorescence. On day 28, all rats were killed to examine NET formation in the peritoneum and the development of AAV. Results: IVIG-S significantly inhibited NET formation induced by PMA in vitro. NET amounts in the peritoneum in Group 1 were significantly smaller than in Group 2, and ANCA titers in Group 1 were significantly lower than in Group 2. The degree of pulmonary hemorrhage in Group 1 was also smaller than in Group 2. Conclusion: Pharmaceutical immunoglobulins reduce NET formation and ameliorate the development of MPO-AAV.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is an Accepted Manuscript of an article published by Taylor & Francis in Modern Rheumatology on 12 Apr 2019, available online: http://www.tandfonline.com/10.1080/14397595.2019.1602292.
PISSN	1439-7595
EISSN	1439-7609
NCID	AA1157187X
関連情報	PMID 30932727
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1080/14397595.2019.1602292