Bone marrow mesenchymal stem cells combined with ultra-purified alginate gel as a regenerative therapeutic strategy after discectomy for degenerated intervertebral discs

Ukeba, Daisuke; Sudo, Hideki; Tsujimoto, Takeru; Ura, Katsuro; Yamada, Katsuhisa; Iwasaki, Norimasa

Bone marrow mesenchymal stem cells combined with ultra-purified alginate gel as a regenerative therapeutic strategy after discectomy for degenerated intervertebral discs

Ukeba, Daisuke; Sudo, Hideki; Tsujimoto, Takeru et al.

EBioMedicine, 2020, 53, UNSP 102698

Permalink : https://hdl.handle.net/2115/78194

このアイテムのアクセス数:562件（2026-10-08 00:34 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/78194
タイトル	Bone marrow mesenchymal stem cells combined with ultra-purified alginate gel as a regenerative therapeutic strategy after discectomy for degenerated intervertebral discs
著者	Ukeba, Daisuke ; Sudo, Hideki ; Tsujimoto, Takeru ; Ura, Katsuro ; Yamada, Katsuhisa ; Iwasaki, Norimasa
著者名 Ukeba, Daisuke
e-Rad_Researcher 30374367 著者名 Sudo, Hideki
著者名 Tsujimoto, Takeru
著者名 Ura, Katsuro
著者名 Yamada, Katsuhisa
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
言語	英語
キーワード	Intervertebral disc
	Herniated disc
	Bone marrow mesenchymal stem cell
	Biomaterial
発行日	2020-03
出版者	Elsevier
収録物名	EBioMedicine
巻(号)	53
ページ	UNSP 102698
抄録	Background: Because the regenerative ability of intervertebral discs (IVDs) is restricted, defects caused by discectomy may induce insufficient tissue repair leading to further IVD degeneration. An acellular bioresorbable biomaterial based on ultra-purified alginate (UPAL) gel was developed to fill the IVD cavity and prevent IVD degeneration. However, an acellular matrix-based strategy may have limitations, particularly in the elderly population, who exhibit low self-repair capability. Therefore, further translational studies involving product combinations, such as UPAL gel plus bone marrow-derived mesenchymal stem cells (BMSCs), are required to evaluate the regenerative effects of BMSCs embedded in UPAL gel on degenerated IVDs. Methods: Rabbit BMSCs and nucleus pulposus cells (NPCs) were co-cultured in a three-dimensional (3D) system in UPAL gel. In addition, rabbit or human BMSCs combined with UPAL gel were implanted into IVDs following partial discectomy in rabbits with degenerated IVDs. Findings: Gene expression of NPC markers, growth factors, and extracellular matrix was significantly increased in the NPC and BMSC 3D co-culture compared to that in each 3D mono-culture. In vivo, whereas UPAL gel alone suppressed IVD degeneration as compared to discectomy, the combination of BMSCs and UPAL gel exerted a more potent effect to induce IVD regeneration. Similar IVD regeneration was observed using human BMSCs. Interpretation: These findings demonstrate the therapeutic potential of BMSCs combined with UPAL gel as a regenerative strategy following discectomy for degenerated IVDs. Funding: Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd. (c) 2020 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	2352-3964
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.ebiom.2020.102698