STAP-2 positively regulates Fc epsilon RI-mediated basophil activation and basophil-dependent allergic inflammatory reactions

Kashiwakura, Jun-ichi; Yamashita, Shinsuke; Yoshihara, Mari; Inui, Kyosuke; Saitoh, Kodai; Sekine, Yuichi; Muromoto, Ryuta; Kitai, Yuichi; Oritani, Kenji; Matsuda, Tadashi

STAP-2 positively regulates Fc epsilon RI-mediated basophil activation and basophil-dependent allergic inflammatory reactions

Kashiwakura, Jun-ichi; Yamashita, Shinsuke; Yoshihara, Mari et al.

International immunology, 2019, 31(5), 349-356

Permalink : https://hdl.handle.net/2115/78248

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アクセス権	open access
URI	https://hdl.handle.net/2115/78248
タイトル	STAP-2 positively regulates Fc epsilon RI-mediated basophil activation and basophil-dependent allergic inflammatory reactions
著者	Kashiwakura, Jun-ichi ; Yamashita, Shinsuke ; Yoshihara, Mari ; Inui, Kyosuke ; Saitoh, Kodai ; Sekine, Yuichi ; Muromoto, Ryuta ; Kitai, Yuichi ; Oritani, Kenji ; Matsuda, Tadashi
e-Rad_Researcher 90373290 著者名 Kashiwakura, Jun-ichi
著者名 Yamashita, Shinsuke
著者名 Yoshihara, Mari
著者名 Inui, Kyosuke
著者名 Saitoh, Kodai
著者名 Sekine, Yuichi
e-Rad_Researcher 30455597 著者名 Muromoto, Ryuta
e-Rad_Researcher 90756165 著者名 Kitai, Yuichi
著者名 Oritani, Kenji
e-Rad_Researcher 20212219 著者名 Matsuda, Tadashi
言語	英語
キーワード	basophils
	IgE
	IgE-CAI
	IL-4
	STAP-2
発行日	2019-05
出版者	Oxford University Press
収録物名	International immunology
巻(号)	31(5)
ページ	349-356
抄録	Basophils are an important cell type in the regulation of T(h)2 immune responses. Recently, we revealed that signal-transducing adaptor protein-2 (STAP-2) negatively regulates mast cell activation via Fc epsilon RI. However, the role of STAP-2 in basophil maturation and activation remained unclear. In this study, we demonstrated the normal development of basophils in STAP-2-deficient (STAP-2(-/-)) mice. We also demonstrated in vitro normal basophil differentiation and FceRI expression in STAP2(-/-) mice, suggesting that STAP-2 is dispensable for basophil maturation. Using bone marrow-derived cultured basophils (BMBs), we showed that degranulation and cytokine production of STAP-2(-/-) BMBs were lower than those of wild-type (WT) BMBs upon stimulation with IgE/Ag. In accordance with the reduction of degranulation and cytokine production, phosphorylation of several signal molecules such as Lyn, PLC-gamma 2 and Erk was reduced in STAP-2(-/-) BMBs after stimulation via Fc epsilon RI. Finally, it was observed that IgE-dependent chronic allergic inflammation of STAP-2(-/-) mice was significantly inhibited compared with WT mice. Taken together, we conclude that STAP-2 is an adaptor molecule that positively regulates FceRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced version of an article accepted for publication in International Immunology following peer review. The version of record Jun-ichi Kashiwakura et al. STAP-2 positively regulates FcεRI-mediated basophil activation and basophil-dependent allergic inflammatory reactions. International Immunology, Volume 31, Issue 5, May 2019, Pages 349–356. is available online at: https://doi.org/10.1093/intimm/dxz013
PISSN	0953-8178
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/intimm/dxz013