Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel

Hontani, Kazutoshi; Onodera, Tomohiro; Terashima, Michiyo; Momma, Daisuke; Matsuoka, Masatake; Baba, Rikiya; Joutoku, Zenta; Matsubara, Shinji; Homan, Kentaro; Hishimura, Ryosuke; Xu, Liang; Iwasaki, Norimasa

Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel

Hontani, Kazutoshi; Onodera, Tomohiro; Terashima, Michiyo et al.

Journal of biomedical materials research part A, 2019, 107(5), 1086-1093

Permalink : https://hdl.handle.net/2115/78388

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アクセス権	open access
URI	https://hdl.handle.net/2115/78388
タイトル	Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel
その他のタイトル	Chondrogenic differentiation of mouse induced pluripotent stem cells (iPSCs) using the 3 three-dimensional culture with ultra-purified alginate gel (UPAL gel)
著者	Hontani, Kazutoshi ; Onodera, Tomohiro ; Terashima, Michiyo ; Momma, Daisuke ; Matsuoka, Masatake ; Baba, Rikiya ; Joutoku, Zenta ; Matsubara, Shinji ; Homan, Kentaro ; Hishimura, Ryosuke ; Xu, Liang ; Iwasaki, Norimasa
e-Rad_Researcher 10805279 著者名 Hontani, Kazutoshi
e-Rad_Researcher 70547174 著者名 Onodera, Tomohiro
著者名 Terashima, Michiyo
e-Rad_Researcher 50779866 著者名 Momma, Daisuke
e-Rad_Researcher 70816066 著者名 Matsuoka, Masatake
e-Rad_Researcher 40815742 著者名 Baba, Rikiya
著者名 Joutoku, Zenta
著者名 Matsubara, Shinji
e-Rad_Researcher 40823331 著者名 Homan, Kentaro
著者名 Hishimura, Ryosuke
著者名 Xu, Liang
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
言語	英語
キーワード	Cartilage repair
	iPS cells
	Mesenchymal stem cells
	Alginate gel
	Chondrogenic differentiation
発行日	2019-05
出版者	John Wiley & Sons
収録物名	Journal of biomedical materials research part A
巻(号)	107(5)
ページ	1086-1093
抄録	As articular cartilages have rarely healed by themselves because of their characteristics of avascularity and low cell density, surgical intervention is ideal for patients with cartilaginous injuries. Because of structural characteristics of the cartilage tissue, a three-dimensional culture of stem cells in biomaterials is a favorable system on cartilage tissue engineering. Induced pluripotent stem cells (iPSCs) are a new cell source in cartilage tissue engineering for its characteristics of self-renewal capability and pluripotency. However, the optimal cultivation condition for chondrogenesis of iPSCs is still unknown. Here we show that a novel chondrogenic differentiation method of iPSCs using the combination of three-dimensional cultivation in ultra-purified alginate gel (UPAL gel) and multi-step differentiation via mesenchymal stem cell-like cells (iPS-MSCs) could efficiently and specifically differentiate iPSCs into chondrocytes. The iPS-MSCs in UPAL gel culture sequentially enhanced the expression of chondrogenic marker without the upregulation of that of osteogenic and adipogenic marker and histologically showed homogeneous chondrogenic extracellular matrix formation. Our results suggest that the pluripotency of iPSCs can be controlled when iPSCs are differentiated into iPS-MSCs before embedding in UPAL gel. These results lead to the establishment of an efficient three-dimensional system to engineer artificial cartilage tissue from iPSCs for cartilage regeneration. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1086-1093, 2019.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: Kazutoshi Hontani, Tomohiro Onodera, Michiyo Terashima, Daisuke Momma, Masatake Matsuoka Rikiya Baba, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Ryosuke Hishimura, Liang Xu, Norimasa Iwasaki (2018) Chondrogenic differentiation of mouse induced pluripotent stem cells using the three‐dimensional culture with ultra‐purified alginate gel. J. Biomed. Mater. Res. Part A, 88: 1086–1093, which has been published in final form at https://doi.org/10.1002/jbm.a.36615. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	1549-3296
EISSN	1552-4965
NCID	AA11881516
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/jbm.a.36615