Isolation of Rickettsia, Rickettsiella, and Spiroplasma from Questing Ticks in Japan Using Arthropod Cells

Thu, May June; Qiu, Yongjin; Kataoka-Nakamura, Chikako; Sugimoto, Chihiro; Katakura, Ken; Isoda, Norikazu; Nakao, Ryo

Isolation of Rickettsia, Rickettsiella, and Spiroplasma from Questing Ticks in Japan Using Arthropod Cells

Thu, May June; Qiu, Yongjin; Kataoka-Nakamura, Chikako et al.

Vector-Borne and Zoonotic Diseases, 2019, 19(7), 474-485

Permalink : https://hdl.handle.net/2115/78725

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アクセス権	open access
URI	https://hdl.handle.net/2115/78725
タイトル	Isolation of Rickettsia, Rickettsiella, and Spiroplasma from Questing Ticks in Japan Using Arthropod Cells
著者	Thu, May June ; Qiu, Yongjin ; Kataoka-Nakamura, Chikako ; Sugimoto, Chihiro ; Katakura, Ken ; Isoda, Norikazu ; Nakao, Ryo
著者名 Thu, May June
著者名 Qiu, Yongjin
著者名 Kataoka-Nakamura, Chikako
著者名 Sugimoto, Chihiro
著者名 Katakura, Ken
著者名 Isoda, Norikazu
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
言語	英語
キーワード	arthropods
	isolation
	Rickettsia
	Rickettsiella
	Spiroplasma
	symbionts
発行日	2019-06
出版者	Mary Ann Liebert
収録物名	Vector-Borne and Zoonotic Diseases
巻(号)	19(7)
ページ	474-485
抄録	Ticks are blood-sucking ectoparasites that transmit zoonotic pathogens to humans and animals. Ticks harbor not only pathogenic microorganisms but also endosymbionts. Although some tick endosymbionts are known to be essential for the survival of ticks, their roles in ticks remain poorly understood. The main aim of this study was to isolate and characterize tick-borne microorganisms from field-collected ticks using two arthropod cell lines derived from Ixodes scapularis embryos (ISE6) and Aedes albopictus larvae (C6/36). A total of 170 tick homogenates originating from 15 different tick species collected in Japan were inoculated into each cell line. Bacterial growth was confirmed by PCR amplification of 16S ribosomal DNA (rDNA) of eubacteria. During the 8-week observation period, bacterial isolation was confirmed in 14 and 4 samples using ISE6 and C6/36 cells, respectively. The sequencing analysis of the 16S rDNA PCR products indicated that they were previously known tick-borne pathogens/endosymbionts in three different genera: Rickettsia, Rickettsiella, and Spiroplasma. These included four previously validated rickettsial species namely Rickettsia asiatica (n = 2), Rickettsia helvetica (n = 3), Rickettsia monacensis (n = 2), and Rickettsia tamurae (n = 3) and one uncharacterized genotype Rickettsia sp. LON (n = 2). Four isolates of Spiroplasma had the highest similarity with previously reported Spiroplasma isolates: Spiroplasma ixodetis obtained from ticks in North America and Spiroplasma sp. Bratislava 1 obtained from Ixodes ricinus in Europe, while two isolates of Rickettsiella showed 100% identity with Rickettsiella sp. detected from Ixodes uriae at Grimsey Island in Iceland. To the best of our knowledge, this is the first report on successful isolation of Rickettsiella from ticks. The isolates obtained in this study can be further analyzed to evaluate their pathogenic potential in animals and their roles as symbionts in ticks.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/vbz.2018.2373
PISSN	1530-3667
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1089/vbz.2018.2373