Advantages of Using Paclitaxel in Combination with Oncolytic Adenovirus Utilizing RNA Destabilization Mechanism

Hossain, Elora; Habiba, Umma; Yanagawa-Matsuda, Aya; Alam, Arefin; Ahmed, Ishraque; Alam, Mohammad Towfik; Yasuda, Motoaki; Higashino, Fumihiro

Advantages of Using Paclitaxel in Combination with Oncolytic Adenovirus Utilizing RNA Destabilization Mechanism

Hossain, Elora; Habiba, Umma; Yanagawa-Matsuda, Aya et al.

Cancers, 2020, 12(5), 1210

Permalink : https://hdl.handle.net/2115/78874

このアイテムのアクセス数:651件（2026-08-13 22:23 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/78874
タイトル	Advantages of Using Paclitaxel in Combination with Oncolytic Adenovirus Utilizing RNA Destabilization Mechanism
著者	Hossain, Elora ; Habiba, Umma ; Yanagawa-Matsuda, Aya ; Alam, Arefin ; Ahmed, Ishraque ; Alam, Mohammad Towfik ; Yasuda, Motoaki ; Higashino, Fumihiro
著者名 Hossain, Elora
e-Rad_Researcher 40755914 著者名 Habiba, Umma
e-Rad_Researcher 60514312 著者名 Yanagawa-Matsuda, Aya
著者名 Alam, Arefin
著者名 Ahmed, Ishraque
e-Rad_Researcher 60641694 著者名 Alam, Mohammad Towfik
e-Rad_Researcher 90239765 著者名 Yasuda, Motoaki
e-Rad_Researcher 50301891 著者名 Higashino, Fumihiro
言語	英語
キーワード	Oncolytic adenovirus
	E1A
	AU-rich element (ARE)
	HuR
	ARE-mRNA
	Paclitaxel
	Microtubules
	CAR
発行日	2020-05
出版者	MDPI
収録物名	Cancers
巻(号)	12(5)
ページ	1210
抄録	Oncolytic virotherapy is a novel approach to cancer therapy. Ad-fosARE is a conditionally replicative adenovirus engineered by inserting AU-rich elements (ARE) in the 3'-untranslated region of the E1A gene. In this study, we examined the oncolytic activity of Ad-fosARE and used it in a synergistic combination with the chemotherapeutic agent paclitaxel (PTX) for treating cancer cells. The expression of E1A was high in cancer cells due to stabilized E1A-ARE mRNA. As a result, the efficiency of its replication and cytolytic activity in cancer cells was higher than in normal cells. PTX treatment increased the cytoplasmic HuR relocalization in cancer cells, enhanced viral replication through elevated E1A expression, and upregulated CAR (Coxsackie-adenovirus receptor) required for viral uptake. Furthermore, PTX altered the instability of microtubules by acetylation and detyrosination, which is essential for viral internalization and trafficking to the nucleus. These results indicate that PTX can provide multiple advantages to the efficacy of Ad-fosARE both in vitro and in vivo, and provides a basis for designing novel clinical trials. Thus, this virus has a lot of benefits that are not found in other oncolytic viruses. The virus also has the potential for treating PXT-resistant cancers.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/cancers12051210