A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose

Togo, Yuki; Nakashima, Kazunori; Mwandira, Wilson et al.

Analytical sciences, 2020, 36(4), 459-464

Permalink : https://hdl.handle.net/2115/78992

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https://doi.org/10.2116/analsci.19P356     15

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アクセス権
open access
URI
https://hdl.handle.net/2115/78992
タイトル
A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose
著者
Togo, Yuki ; Nakashima, Kazunori ; Mwandira, Wilson ; Kawasaki, Satoru
著者名
Togo, Yuki
著者名
Nakashima, Kazunori
著者名
Mwandira, Wilson
e-Rad_Researcher
00304022
著者名
Kawasaki, Satoru
言語
英語
キーワード
Carbohydrate-binding module
immobilization
metal affinity tag
transition metals
発行日
2020-04
出版者
日本分析化学会（Japan Society for Analytical Chemistry）
収録物名
Analytical sciences
巻(号)
36(4)
ページ
459-464
抄録
We developed a novel metal adsorbent composed of bio-based materials, cellulose and a protein. The approach involved the immobilization of a hexa-histidine tag (His(6)), which shows an affinity for an intermediate acid (metal ion) in Hard and Soft Acids and Bases (HSAB) theory, on cellulose by fusing with a carbohydrate-binding module (CBM). The results show that CBM-His(6)-bound cellulose has adsorption selectivity reflecting the original properties of His(6). Additionally, we prepared three configurations of CBM-His(6) proteins, which were subsequently immobilized on filter paper for Ni2+ ion adsorption. Of these configurations, we found that the protein containing two His(6) tags at each terminus (N- and C-) of CBM exhibited the highest metal adsorption ability. Furthermore, XPS analysis confirmed the binding of Ni2+ ions on the cellulose.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0910-6340
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.2116/analsci.19P356