Quantifying Protein-Specific N-Glycome Profiles by Focused Protein and Immunoprecipitation Glycomics

Kobayashi, Takashi; Ogawa, Koji; Furukawa, Jun-ichi; Hanamatsu, Hisatoshi; Hato, Megumi; Yoshinaga, Tomoyo; Morikawa, Kenichi; Suda, Goki; Sho, Takuya; Nakai, Masato; Higashino, Kenichi; Numata, Yoshito; Shinohara, Yasuro; Sakamoto, Naoya

Quantifying Protein-Specific N-Glycome Profiles by Focused Protein and Immunoprecipitation Glycomics

Kobayashi, Takashi; Ogawa, Koji; Furukawa, Jun-ichi et al.

Journal of proteome research, 2019, 18(8), 3133-3141

Permalink : https://hdl.handle.net/2115/79019

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アクセス権	open access
URI	https://hdl.handle.net/2115/79019
タイトル	Quantifying Protein-Specific N-Glycome Profiles by Focused Protein and Immunoprecipitation Glycomics
著者	Kobayashi, Takashi ; Ogawa, Koji ; Furukawa, Jun-ichi ; Hanamatsu, Hisatoshi ; Hato, Megumi ; Yoshinaga, Tomoyo ; Morikawa, Kenichi ; Suda, Goki ; Sho, Takuya ; Nakai, Masato ; Higashino, Kenichi ; Numata, Yoshito ; Shinohara, Yasuro ; Sakamoto, Naoya
著者名 Kobayashi, Takashi
e-Rad_Researcher 20735188 著者名 Ogawa, Koji
e-Rad_Researcher 30374193 著者名 Furukawa, Jun-ichi
e-Rad_Researcher 70734185 著者名 Hanamatsu, Hisatoshi
著者名 Hato, Megumi
著者名 Yoshinaga, Tomoyo
著者名 Morikawa, Kenichi
e-Rad_Researcher 20447460 著者名 Suda, Goki
著者名 Sho, Takuya
著者名 Nakai, Masato
著者名 Higashino, Kenichi
著者名 Numata, Yoshito
著者名 Shinohara, Yasuro
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	biomarker
	glycomics
	fucosylation
	alpha-1 antitrypsin
	ceruloplasmin
	NASH
	MALDI-TOF MS
発行日	2019-08
出版者	American Chemical Society
収録物名	Journal of proteome research
巻(号)	18(8)
ページ	3133-3141
抄録	Serum N-glycans have been reported to be potential diagnostic and therapeutic biomarkers for many diseases and conditions, such as inflammation, fibrosis, and cancer progression. We previously described the focused protein glycomic analysis (FPG) from gel-separated serum proteins. With this methodology, we sought novel glycan biomarkers for nonalcoholic steatohepatitis (NASH) and successfully identified some N-glycans that were significantly elevated in NASH patients compared to nonalcoholic fatty liver patients. Among them, trisialylated monofucosylated triantennary glycan (A3F) of alpha-1 antitrypsin showed the most dynamic change. For rapid identification of N-glycans on the focused proteins, we constructed a simplified method called immunoprecipitation glycomics (IPG), where the target proteins were immunoprecipitated with affinity beads and subsequently subjected to glycomic analysis by MALDI-TOF MS. Focusing on alpha-1 antitrypsin and ceruloplasmin as the target proteins, we compared the values of N-glycans determined by FPG and IPG. The quantified values of each N-glycan by these two methods showed a statistically significant correlation, indicating that high throughput and quantitative N-glycomics of targeted proteins can be achieved by the simplified IPG method. Thus, an analytical strategy combining FPG and IPG can be adapted to general biomarker discovery and validation in appropriate disease areas.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of proteome research, copyright c American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jproteome.9b00232.
PISSN	1535-3893
関連情報	PMID 31266306
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1021/acs.jproteome.9b00232