LncRNA-dependent nuclear stress bodies promote intron retention through SR protein phosphorylation

Ninomiya, Kensuke; Adachi, Shungo; Natsume, Tohru; Iwakiri, Junichi; Terai, Goro; Asai, Kiyoshi; Hirose, Tetsuro

LncRNA-dependent nuclear stress bodies promote intron retention through SR protein phosphorylation

Ninomiya, Kensuke; Adachi, Shungo; Natsume, Tohru et al.

EMBO Journal, 2020, 39(23), e102729

Permalink : https://hdl.handle.net/2115/79022

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アクセス権	open access
URI	https://hdl.handle.net/2115/79022
タイトル	LncRNA-dependent nuclear stress bodies promote intron retention through SR protein phosphorylation
著者	Ninomiya, Kensuke ; Adachi, Shungo ; Natsume, Tohru ; Iwakiri, Junichi ; Terai, Goro ; Asai, Kiyoshi ; Hirose, Tetsuro
e-Rad_Researcher 00437279 著者名 Ninomiya, Kensuke
著者名 Adachi, Shungo
e-Rad_Researcher 00357683 著者名 Natsume, Tohru
e-Rad_Researcher 40770160 著者名 Iwakiri, Junichi
著者名 Terai, Goro
e-Rad_Researcher 30356357 著者名 Asai, Kiyoshi
e-Rad_Researcher 30273220 著者名 Hirose, Tetsuro
言語	英語
キーワード	intron retention
	noncoding RNA
	nuclear stress bodies
	phosphorylation
	splicing factors
発行日	2020-02-03
出版者	John Wiley & Sons
収録物名	EMBO Journal
巻(号)	39(23)
ページ	e102729
抄録	A number of long noncoding RNAs (lncRNAs) are induced in response to specific stresses to construct membrane-less nuclear bodies; however, their function remains poorly understood. Here, we report the role of nuclear stress bodies (nSBs) formed on highly repetitive satellite III (HSATIII) lncRNAs derived from primate-specific satellite III repeats upon thermal stress exposure. A transcriptomic analysis revealed that depletion of HSATIII lncRNAs, resulting in elimination of nSBs, promoted splicing of 533 retained introns during thermal stress recovery. A HSATIII-Comprehensive identification of RNA-binding proteins by mass spectrometry (ChIRP-MS) analysis identified multiple splicing factors in nSBs, including serine and arginine-rich pre-mRNA splicing factors (SRSFs), the phosphorylation states of which affect splicing patterns. SRSFs are rapidly de-phosphorylated upon thermal stress exposure. During stress recovery, CDC like kinase 1 (CLK1) was recruited to nSBs and accelerated the re-phosphorylation of SRSF9, thereby promoting target intron retention. Our findings suggest that HSATIII-dependent nSBs serve as a conditional platform for phosphorylation of SRSFs by CLK1 to promote the rapid adaptation of gene expression through intron retention following thermal stress exposure.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: The EMBO Journal: 39(3): e102729., which has been published in final form at https://doi.org/10.15252/embj.2019102729. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	0261-4189
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.15252/embj.2019102729