Genetic Diversity and Sequence Polymorphism of Two Genes Encoding Theileria parva Antigens Recognized by CD8(+) T Cells among Vaccinated and Unvaccinated Cattle in Malawi

Chatanga, Elisha; Hayashida, Kyoko; Muleya, Walter; Kusakisako, Kodai; Moustafa, Mohamed Abdallah Mohamed; Salim, Bashir; Katakura, Ken; Sugimoto, Chihiro; Nonaka, Nariaki; Nakao, Ryo

Genetic Diversity and Sequence Polymorphism of Two Genes Encoding Theileria parva Antigens Recognized by CD8(+) T Cells among Vaccinated and Unvaccinated Cattle in Malawi

Chatanga, Elisha; Hayashida, Kyoko; Muleya, Walter et al.

Pathogens, 2020, 9(5), 334

Permalink : https://hdl.handle.net/2115/79032

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アクセス権	open access
URI	https://hdl.handle.net/2115/79032
タイトル	Genetic Diversity and Sequence Polymorphism of Two Genes Encoding Theileria parva Antigens Recognized by CD8(+) T Cells among Vaccinated and Unvaccinated Cattle in Malawi
著者	Chatanga, Elisha ; Hayashida, Kyoko ; Muleya, Walter ; Kusakisako, Kodai ; Moustafa, Mohamed Abdallah Mohamed ; Salim, Bashir ; Katakura, Ken ; Sugimoto, Chihiro ; Nonaka, Nariaki ; Nakao, Ryo
著者名 Chatanga, Elisha
著者名 Hayashida, Kyoko
著者名 Muleya, Walter
著者名 Kusakisako, Kodai
著者名 Moustafa, Mohamed Abdallah Mohamed
著者名 Salim, Bashir
著者名 Katakura, Ken
著者名 Sugimoto, Chihiro
著者名 Nonaka, Nariaki
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
言語	英語
キーワード	Malawi
	Theileria parva
	genetic diversity
	vaccine
	Muguga cocktail
発行日	2020-05
出版者	MDPI
収録物名	Pathogens
巻(号)	9(5)
ページ	334
抄録	East Coast fever (ECF) is an acute fatal tick-borne disease of cattle caused by Theileria parva. It causes major losses in exotic and crossbreed cattle, but this could be prevented by a vaccine of T. parva if the vaccine is selected properly based on information from molecular epidemiology studies. The Muguga cocktail (MC) vaccine (Muguga, Kiambu 5 and Serengeti-transformed strains) has been used on exotic and crossbreed cattle. A total of 254 T. parva samples from vaccinated and unvaccinated cattle were used to understand the genetic diversity of T. parva in Malawi using partial sequences of the Tp1 and Tp2 genes encoding T. parva CD8(+) antigens, known to be immunodominant and current candidate antigens for a subunit vaccine. Single nucleotide polymorphisms were observed at 14 positions (3.65%) in Tp1 and 156 positions (33.12%) in Tp2, plus short deletions in Tp1, resulting in 6 and 10 amino acid variants in the Tp1 and Tp2 genes, respectively. Most sequences were either identical or similar to T. parva Muguga and Kiambu 5 strains. This may suggest the possible expansion of vaccine components into unvaccinated cattle, or that a very similar genotype already existed in Malawi. This study provides information that support the use of MC to control ECF in Malawi.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/pathogens9050334