Phosphorylated chitin increased bone formation when implanted into rat calvaria with the Ti-device

Song, Qin; Yagami, Kimitoshi; Furusawa, Toshitake; Takita, Hiroko; Kurasaki, Masaaki; Tokura, Seiichi; Morimoto, Koichi; Sammons, Rachel; Iku, Shouhei; Kuboki, Yoshinori

Phosphorylated chitin increased bone formation when implanted into rat calvaria with the Ti-device

Song, Qin; Yagami, Kimitoshi; Furusawa, Toshitake et al.

Bio-medical materials and engineering, 2020, 31(1), 47-57

Permalink : https://hdl.handle.net/2115/79046

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アクセス権	open access
URI	https://hdl.handle.net/2115/79046
タイトル	Phosphorylated chitin increased bone formation when implanted into rat calvaria with the Ti-device
著者	Song, Qin ; Yagami, Kimitoshi ; Furusawa, Toshitake ; Takita, Hiroko ; Kurasaki, Masaaki ; Tokura, Seiichi ; Morimoto, Koichi ; Sammons, Rachel ; Iku, Shouhei ; Kuboki, Yoshinori
著者名 Song, Qin
著者名 Yagami, Kimitoshi
著者名 Furusawa, Toshitake
e-Rad_Researcher 30125330 著者名 Takita, Hiroko
e-Rad_Researcher 80161727 著者名 Kurasaki, Masaaki
e-Rad_Researcher 40000806 著者名 Tokura, Seiichi
著者名 Morimoto, Koichi
著者名 Sammons, Rachel
著者名 Iku, Shouhei
e-Rad_Researcher 00014001 著者名 Kuboki, Yoshinori
言語	英語
キーワード	Chitin
	phosphorylation
	enhanced bone formation
	Ti-device
発行日	2020-05-12
出版者	IOS Press
収録物名	Bio-medical materials and engineering
巻(号)	31(1)
ページ	47-57
抄録	BACKGROUND: Previously we found that a group of phosphorylated proteins (SIBLINGs) in bone binds with the Ti-device, and increases the early bone formation around the Ti-implants remarkably. From these results, we explained the biochemical mechanism of a strong bond between living bone and Ti, which was discovered by Branemark and colleagues. For the clinical application of our findings, we need a large amount of these proteins or their substitutes. OBJECTIVE: We aimed to create a new molecule that equips with essential functions of SIBLINGs, Ti-binding, and bone enhancement around the Ti implant. METHODS: We chemically phosphorylated chitin and obtained a soluble form of phosphorylated chitin (P-chitin). In this solution, we immersed the Ti-devices of web-form (TW) which we previously developed and obtained the P-chitin coated TWs. Then we tested the P-chitin coated TWs for their calcification ability in vitro, and bone enhancing ability in vivo, by implanting them into rat calvaria. We compared the P-chitin coated TW and the non-coated TW in regard to their calcification and bone enhancing abilities. RESULTS: Ti-devices coated with phosphorylated-chitin induced a ten times higher calcification in vitro at 20 days, and four times more elevated amount of bone formation in vivo at two weeks than the uncoated Ti-device. CONCLUSIONS: Phosphorylated chitin could be a partial substitute of bone SIBLING proteins and are clinically applicable to accelerate bone formation around the Ti implants, thereby achieving the strong bond between living bone and Ti.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at IOS Press through http://dx.doi.org/10.3233/BME-201079
PISSN	0959-2989
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.3233/BME-201079