Interaction between titanium and phosphoproteins revealed by chromatography column packed with titanium beads

Kuboki, Yoshinori; Furusawa, Toshitake; Sato, Masaaki; Sun, Yongkun; Unuma, Hidero; Fujisawa, Ryuichi; Abe, Shigeaki; Akasaka, Tsukasa; Watari, Fumio; Takita, Hiroko; Sammons, Rachel

Interaction between titanium and phosphoproteins revealed by chromatography column packed with titanium beads

Kuboki, Yoshinori; Furusawa, Toshitake; Sato, Masaaki et al.

Bio-Medical Materials and Engineering, 2012, 22(5), 283-288

Permalink : https://hdl.handle.net/2115/79048

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アクセス権	open access
URI	https://hdl.handle.net/2115/79048
タイトル	Interaction between titanium and phosphoproteins revealed by chromatography column packed with titanium beads
著者	Kuboki, Yoshinori ; Furusawa, Toshitake ; Sato, Masaaki ; Sun, Yongkun ; Unuma, Hidero ; Fujisawa, Ryuichi ; Abe, Shigeaki ; Akasaka, Tsukasa ; Watari, Fumio ; Takita, Hiroko ; Sammons, Rachel
e-Rad_Researcher 00014001 著者名 Kuboki, Yoshinori
著者名 Furusawa, Toshitake
著者名 Sato, Masaaki
著者名 Sun, Yongkun
著者名 Unuma, Hidero
著者名 Fujisawa, Ryuichi
e-Rad_Researcher 40374566 著者名 Abe, Shigeaki
e-Rad_Researcher 00360917 著者名 Akasaka, Tsukasa
e-Rad_Researcher 70158682 著者名 Watari, Fumio
e-Rad_Researcher 30125330 著者名 Takita, Hiroko
著者名 Sammons, Rachel
言語	英語
キーワード	Titanium binding proteins
	chromatography
	phosphoprotein
	caseins
	phosvitin
	dentin phosphoprotein
発行日	2012
出版者	IOS Press
収録物名	Bio-Medical Materials and Engineering
巻(号)	22(5)
ページ	283-288
抄録	The biochemical mechanism behind the strong binding between titanium and living bone has not been fully elucidated, in spite of worldwide clinical application of this phenomenon. We hypothesized that one of the core mechanisms may reside in the interaction between certain proteins in the host tissues and the implanted titanium. To verify the interaction between titanium and proteins, we chose the technique of chromatography in that titanium spherical beads (45 μm) were packed into a column to obtain a bed volume of 16×50 mm, which was eluted with phosphate buffered saline (PBS) and a straight gradient system made by using PBS and 25 mM NaOH. Fetal calf serum, albumin, lysozyme, casein, phosvitin and dentin phosphoprotein (phosphophoryn) were applied to the column. Most part of albumin and lysozyme eluted with the breakthrough peak, indicating practically no affinity to titanium. Fetal bovine serum also eluted mostly as the breakthrough peak, but distinct retained peak was observed. On the other hand, α-casein, phosvitin and phosphophoryn exhibited a distinct retained peak separated from the breakthrough peak. We proposed that phosphate groups (phosphoserines) in the major phosphoproteins, α-casein, phosvitin and phosphophoryn may be involved in the binding of these proteins with titanium.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at IOS Press through http://dx.doi.org/10.3233/BME-2012-0718
PISSN	0959-2989
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.3233/BME-2012-0718