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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/79063
タイトル	Highly sensitive detection of clenbuterol in urine sample by using surface plasmon resonance immunosensor
著者	Kabiraz, Dulal C. ; Morita, Kinichi ; Sakamoto, Kazuhira ; Takahashi, Masashi ; Kawaguchi, Toshikazu
著者名 Kabiraz, Dulal C.
著者名 Morita, Kinichi
著者名 Sakamoto, Kazuhira
e-Rad_Researcher 10343964 著者名 Takahashi, Masashi
e-Rad_Researcher 90448407 著者名 Kawaguchi, Toshikazu
言語	英語
キーワード	Clenbuterol
	SPR immunosensing
	Indirect competitive inhibition
	Inhibitor determination
	Urine
発行日	2018-08-15
出版者	Elsevier
収録物名	Talanta
巻(号)	186
ページ	521-526
抄録	This study proposed the filtration method for removal of inhibitors from real urine samples for immunoassay without centrifuge. Although the inhibitors could not be removed by the physical filtration, the carboxyl group terminated silica effectively removed the inhibitors. In a low pH, antibody formed aggregation due to the protonation. We propose to adjust pH of the sample solution by adding a phosphate buffer solution (pH 7.5). As a result of pretreatment, the SPR immunosensing achieved the SPR signal of 45 mdeg and a low limit of detection with 100 ppq (100 fg mL(-1)).
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0039-9140
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.talanta.2018.04.011

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