Comparative Analyses of the Antiviral Activities of IgG and IgA Antibodies to Influenza A Virus M2 Protein

Okuya, Kosuke; Eguchi, Nao; Manzoor, Rashid; Yoshida, Reiko; Saito, Shinji; Suzuki, Tadaki; Sasaki, Michihito; Saito, Takeshi; Kida, Yurie; Mori-Kajihara, Akina; Miyamoto, Hiroko; Ichii, Osamu; Kajihara, Masahiro; Higashi, Hideaki; Takada, Ayato

Comparative Analyses of the Antiviral Activities of IgG and IgA Antibodies to Influenza A Virus M2 Protein

Okuya, Kosuke; Eguchi, Nao; Manzoor, Rashid et al.

Viruses-Basel, 2020, 12(7), 780

Permalink : https://hdl.handle.net/2115/79171

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アクセス権	open access
URI	https://hdl.handle.net/2115/79171
タイトル	Comparative Analyses of the Antiviral Activities of IgG and IgA Antibodies to Influenza A Virus M2 Protein
著者	Okuya, Kosuke ; Eguchi, Nao ; Manzoor, Rashid ; Yoshida, Reiko ; Saito, Shinji ; Suzuki, Tadaki ; Sasaki, Michihito ; Saito, Takeshi ; Kida, Yurie ; Mori-Kajihara, Akina ; Miyamoto, Hiroko ; Ichii, Osamu ; Kajihara, Masahiro ; Higashi, Hideaki ; Takada, Ayato
著者名 Okuya, Kosuke
著者名 Eguchi, Nao
著者名 Manzoor, Rashid
e-Rad_Researcher 80435966 著者名 Yoshida, Reiko
著者名 Saito, Shinji
e-Rad_Researcher 30527180 著者名 Suzuki, Tadaki
e-Rad_Researcher 70609403 著者名 Sasaki, Michihito
e-Rad_Researcher 40153811 著者名 Saito, Takeshi
著者名 Kida, Yurie
著者名 Mori-Kajihara, Akina
著者名 Miyamoto, Hiroko
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
e-Rad_Researcher 70711894 著者名 Kajihara, Masahiro
e-Rad_Researcher 20311227 著者名 Higashi, Hideaki
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
キーワード	influenza A virus
	matrix 2 protein
	antibody
	IgA
	budding inhibition
	cross-protective immunity
発行日	2020-07-20
出版者	MDPI
収録物名	Viruses-Basel
巻(号)	12(7)
ページ	780
抄録	The influenza A virus (IAV) matrix-2 (M2) protein is an antigenically conserved viral envelope protein that plays an important role in virus budding together with another envelope protein, hemagglutinin (HA). An M2-specific mouse monoclonal IgG antibody, rM2ss23, which binds to the ectodomain of the M2 protein, has been shown to be a non-neutralizing antibody, but inhibits plaque formation of IAV strains. In this study, we generated chimeric rM2ss23 (ch-rM2ss23) IgG and IgA antibodies with the same variable region and compared their antiviral activities. Using gel chromatography, ch-rM2ss23 IgA were divided into three antibody subsets: monomeric IgA (m-IgA), dimeric IgA (d-IgA), and trimeric and tetrameric IgA (t/q-IgA). We found that t/q-IgA had a significantly higher capacity to reduce the plaque size of IAVs than IgG and m-IgA, most likely due to the decreased number of progeny virus particles produced from infected cells. Interestingly, HA-M2 colocalization was remarkably reduced on the infected cell surface in the presence of ch-rM2ss23 antibodies. These results indicate that anti-M2 polymeric IgA restricts IAV budding more efficiently than IgG and suggest a role of anti-M2 IgA in cross-protective immunity to IAVs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1999-4915
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/v12070780