Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli

Matsumoto, Ken'ichiro; Saito, Jun; Yokoo, Toshinori; Hori, Chiaki; Nagata, Akihiro; Kudoh, Yuki; Ooi, Toshihiko; Taguchi, Seiichi

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli

Matsumoto, Ken'ichiro; Saito, Jun; Yokoo, Toshinori et al.

Journal of bioscience and bioengineering, 2019, 128(3), 302-306

Permalink : https://hdl.handle.net/2115/79191

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アクセス権	open access
URI	https://hdl.handle.net/2115/79191
タイトル	Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli
著者	Matsumoto, Ken'ichiro ; Saito, Jun ; Yokoo, Toshinori ; Hori, Chiaki ; Nagata, Akihiro ; Kudoh, Yuki ; Ooi, Toshihiko ; Taguchi, Seiichi
e-Rad_Researcher 80360642 著者名 Matsumoto, Ken'ichiro
著者名 Saito, Jun
著者名 Yokoo, Toshinori
著者名 Hori, Chiaki
著者名 Nagata, Akihiro
著者名 Kudoh, Yuki
e-Rad_Researcher 40223713 著者名 Ooi, Toshihiko
著者名 Taguchi, Seiichi
言語	英語
キーワード	Polyhydroxybutyrate
	Polyglycolic acid
	Carbon fixation
	Biodegradable plastic
	Lactate-polymerizing enzyme
発行日	2019-09
出版者	Society for Bioscience and Bioengineering
収録物名	Journal of bioscience and bioengineering
巻(号)	128(3)
ページ	302-306
抄録	Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (RuBisCO) generates 2-phosphoglycolate (2PG) as one of the metabolites from the Calvin-Benson-Bassham (CBB) cycle. In this study, we focused on the fact that glycolate (GL) derived from 2PG can be incorporated into the bacterial polyhydroxyalkanoate (PHA) as the monomeric constituent by using the evolved PHA synthase (PhaC1(Ps)STQK). In this study, the function of the RuBisCO-mediated pathway for GL-based PHA synthesis was evaluated using Escherichia coli JW2946 with the deletion of glycolate oxidase gene (Delta glcD) as the model system. The genes encoding RuBisCO, phosphoribulokinase and 2PG phosphatase (PGPase) from several photosynthetic bacteria were introduced into E. coli, and the cells were grown on xylose as a sole carbon source. The functional expression of RuBisCO and relevant enzymes was confirmed based on the increases in the intracellular concentrations of RuBP and GL. Next, PHA biosynthetic genes encoding PhaC1(Ps)STQK, propionyl-CoA transferase and 3-hydroxybutyryl(3HB)-CoA-supplying enzymes were introduced. The cells accumulated poly(GL-co-3HB)s with GL fractions of 7.8-15.1 mol%. Among the tested RuBisCOs, Rhodosprium rubrum and Synechococcus elongatus PCC7942 enzymes were effective for P(GL-co-3HB) production as well as higher GL fraction. The heterologous expression of PGPase from Synechocystis sp. PCC6803 and R. rubrum increased GL fraction in the polymer. These results demonstrated that the RuBisCO-mediated pathway is potentially used to produce GL-based PHA in not only E. coli but also in photosynthetic organisms. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1389-1723
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jbiosc.2019.03.002