Alterations in characteristics of canine articular chondrocytes in non-passaged long-term monolayer culture: Matter of differentiation, dedifferentiation and redifferentiation

Akaraphutiporn, Ekkapol; Sunaga, Takafumi; Bwalya, Eugene C.; Echigo, Ryosuke; Okumura, Masahiro

Alterations in characteristics of canine articular chondrocytes in non-passaged long-term monolayer culture: Matter of differentiation, dedifferentiation and redifferentiation

Akaraphutiporn, Ekkapol; Sunaga, Takafumi; Bwalya, Eugene C. et al.

Journal of veterinary medical science, 2020, 82(6), 793-803

Permalink : https://hdl.handle.net/2115/79212

このアイテムのアクセス数:618件（2026-08-18 18:19 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1292/jvms.20-0118			17

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/79212
タイトル	Alterations in characteristics of canine articular chondrocytes in non-passaged long-term monolayer culture: Matter of differentiation, dedifferentiation and redifferentiation
著者	Akaraphutiporn, Ekkapol ; Sunaga, Takafumi ; Bwalya, Eugene C. ; Echigo, Ryosuke ; Okumura, Masahiro
著者名 Akaraphutiporn, Ekkapol
e-Rad_Researcher 90649112 著者名 Sunaga, Takafumi
著者名 Bwalya, Eugene C.
著者名 Echigo, Ryosuke
e-Rad_Researcher 80260397 著者名 Okumura, Masahiro
言語	英語
キーワード	articular chondrocyte
	chondrocyte redifferentiation
	long-term culture
	monolayer culture
	tissue engineering
発行日	2020-06
出版者	公益社団法人日本獣医学会 (The Japanese Society of Veterinary Science)
収録物名	Journal of veterinary medical science
巻(号)	82(6)
ページ	793-803
抄録	This study investigated the effects of culture time on phenotype stability of canine articular chondrocytes (CACs) in non-passaged long-term monolayer culture.Third passage (P3) CACs isolated from four cartilage samples were seeded at three different initial seeding densities (0.2 x 10(4),1.0 x 10(4) and 5.0 x 10(4) cells/cm(2)) and maintained in monolayer condition up to 8 weeks without undergoing subculture after confluence. The characteristic changes of chondrocytes during the culture period were evaluated based on the cell morphology, cell proliferation, glycosaminoglycans (GAGs) content, DNA quantification, mRNA expression and ultrastructure of chondrocytes. Chondrocytes maintained under post-confluence condition exhibited a capability to grow and proliferate up to 4 weeks. Alcian blue staining and Dimethylmethylene blue (DMMB) assay revealed that the extracellular matrix (ECM) synthesis was increased in a time-dependent manner from 2 to 8 weeks. The chondrocyte mRNA expression profile was dramatically affected by prolonged culture time, with a significant downregulation of collagen type 1, whereas the expression of collagen type II, aggrecan, Sox9 and matrix metalloproteinase 13 (MMP-13) were significantly upregulated. In addition, transmission electron microscopy (TEM) result indicated dilation of rough endoplasmic reticulum (RER) in these long-term monolayer cultured chondrocytes. These findings demonstrate that the chondrocytes phenotype could be partially redifferentiated through the spontaneous redifferentiation process in long-term cultures using standard culture medium without the addition of chondrogenic supplements or tissue-culture scaffolds.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-7250
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.20-0118