Induction of macrophage-like immunosuppressive cells from common marmoset ES cells by stepwise differentiation with DZNep

Tsuji, Hyuma; Otsuka, Ryo; Wada, Haruka; Murata, Tomoki; Sasaki, Airi; Itoh, Mizuho; Baghdadi, Muhammad; Sasaki, Erika; Seino, Ken-ichiro

Induction of macrophage-like immunosuppressive cells from common marmoset ES cells by stepwise differentiation with DZNep

Tsuji, Hyuma; Otsuka, Ryo; Wada, Haruka et al.

Scientific reports, 2020, 10(1), 12625

Permalink : https://hdl.handle.net/2115/79260

このアイテムのアクセス数:591件（2026-08-16 23:48 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/79260
タイトル	Induction of macrophage-like immunosuppressive cells from common marmoset ES cells by stepwise differentiation with DZNep
著者	Tsuji, Hyuma ; Otsuka, Ryo ; Wada, Haruka ; Murata, Tomoki ; Sasaki, Airi ; Itoh, Mizuho ; Baghdadi, Muhammad ; Sasaki, Erika ; Seino, Ken-ichiro
著者名 Tsuji, Hyuma
著者名 Otsuka, Ryo
e-Rad_Researcher 70392181 著者名 Wada, Haruka
著者名 Murata, Tomoki
著者名 Sasaki, Airi
著者名 Itoh, Mizuho
e-Rad_Researcher 60711570 著者名 Baghdadi, Muhammad
著者名 Sasaki, Erika
e-Rad_Researcher 20312845 著者名 Seino, Ken-ichiro
言語	英語
発行日	2020-07-28
出版者	Nature Publishing Group
収録物名	Scientific reports
巻(号)	10(1)
ページ	12625
抄録	Recent progress in regenerative medicine has enabled the utilization of pluripotent stem cells (PSCs) as the resource of therapeutic cells/tissue. However, immune suppression is still needed when the donor-recipient combination is allogeneic. We have reported previously that mouse PSCs-derived immunosuppressive cells contribute to prolonged survival of grafts derived from the same mouse PSCs in allogeneic recipients. For its clinical application, a preclinical study using non-human primates such as common marmoset must be performed. In this study, we established the induction protocol of immunosuppressive cells from common marmoset ES cells. Although similar immunosuppressive macrophages could not be induced by same protocol as that for mouse PSCs, we employed an inhibitor for histone methyltransferase, DZNep, and succeeded to induce them. The DZNep-treated macrophage-like cells expressed several immunosuppressive molecules and significantly inhibited allogeneic mixed lymphocyte reaction. The immunosuppressive cells from non-human primate ESCs will help to establish an immunoregulating strategy in regenerative medicine using PSCs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2045-2322
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1038/s41598-020-69690-9