Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater

Ahmed, Warish; Bertsch, Paul M.; Bivins, Aaron et al.

Science of the total environment, 2020, 739, 139960

Permalink : https://hdl.handle.net/2115/79436

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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/79436
タイトル
Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater
著者
Ahmed, Warish ; Bertsch, Paul M. ; Bivins, Aaron ; Bibby, Kyle ; Farkas, Kata ; Gathercole, Amy ; Haramoto, Eiji ; Gyawali, Pradip ; Korajkic, Asja ; McMinn, Brian R. ; Mueller, Jochen F. ; Simpson, Stuart L. ; Smith, Wendy J. M. ; Symonds, Erin M. ; Thomas, Kevin V. ; Verhagen, Rory ; Kitajima, Masaaki
著者名
Ahmed, Warish
著者名
Bertsch, Paul M.
著者名
Bivins, Aaron
著者名
Bibby, Kyle
著者名
Farkas, Kata
著者名
Gathercole, Amy
著者名
Haramoto, Eiji
著者名
Gyawali, Pradip
著者名
Korajkic, Asja
著者名
McMinn, Brian R.
著者名
Mueller, Jochen F.
著者名
Simpson, Stuart L.
著者名
Smith, Wendy J. M.
著者名
Symonds, Erin M.
著者名
Thomas, Kevin V.
著者名
Verhagen, Rory
著者名
Kitajima, Masaaki
言語
英語
キーワード
SARS-CoV-2
COVID-19
Murine hepatitis virus
Recovery
Concentration method
Enveloped virus
Untreated wastewater
Filtration
発行日
2020-10-15
出版者
Elsevier
収録物名
Science of the total environment
巻(号)
739
ページ
139960
抄録
There is currently a clear benefit for many countries to utilize wastewater-based epidemiology (WBE) as part of ongoing measures to manage the coronavirus disease 2019 (COVID-19) global pandemic. Since most wastewater virus concentration methods were developed and validated for nonenveloped viruses, it is imperative to determine the efficiency of the most commonly used methods for the enveloped severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Municipal wastewater seeded with a human coronavirus (CoV) surrogate, murine hepatitis virus (MHV), was used to test the efficiency of seven wastewater virus concentration methods: (A-C) adsorption-extraction with three different pre-treatment options, (D-E) centrifugal filter device methods with two different devices, (F) polyethylene glycol (PEG 8000) precipitation, and (G) ultracentrifugation. MHV was quantified by reverse-transcription quantitative polymerase chain reaction and the recovery efficiency was calculated for each method. The mean MHV recoveries ranged from 26.7 to 65.7%. The most efficient methods were adsorption-extraction methods with MgCl2 pre-treatment (Method C), and without pre-treatment (Method B). The third most efficient method used the Amicon (R) Ultra-15 centrifugal filter device (Method D) and its recovery efficiency was not statistically different from the most efficient methods. The methods with the worst recovery efficiency included the adsorption-extraction method with acidification (A). followed by PEG precipitation (F). Our results suggest that absorption-extraction methods with minimal or without pre-treatment can provide suitably rapid, cost-effective and relatively straightforward recovery of enveloped viruses in wastewater. The MHV is a promising process control for SARS-CoV-2 surveillance and can be used as a quality control measure to support community-level epidemic mitigation and risk assessment.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
権利情報
Creative Commons Attribution 4.0 International
PISSN
0048-9697
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1016/j.scitotenv.2020.139960