Efficient generation of thymic epithelium from induced pluripotent stem cells that prolongs allograft survival

Otsuka, Ryo; Wada, Haruka; Tsuji, Hyuma; Sasaki, Airi; Murata, Tomoki; Itoh, Mizuho; Baghdadi, Muhammad; Seino, Ken-Ichiro

Efficient generation of thymic epithelium from induced pluripotent stem cells that prolongs allograft survival

Otsuka, Ryo; Wada, Haruka; Tsuji, Hyuma et al.

Scientific reports, 2020, 10(1), 224

Permalink : https://hdl.handle.net/2115/79485

このアイテムのアクセス数:532件（2026-11-01 10:19 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/79485
タイトル	Efficient generation of thymic epithelium from induced pluripotent stem cells that prolongs allograft survival
著者	Otsuka, Ryo ; Wada, Haruka ; Tsuji, Hyuma ; Sasaki, Airi ; Murata, Tomoki ; Itoh, Mizuho ; Baghdadi, Muhammad ; Seino, Ken-Ichiro
著者名 Otsuka, Ryo
e-Rad_Researcher 70392181 著者名 Wada, Haruka
著者名 Tsuji, Hyuma
著者名 Sasaki, Airi
著者名 Murata, Tomoki
著者名 Itoh, Mizuho
e-Rad_Researcher 60711570 著者名 Baghdadi, Muhammad
e-Rad_Researcher 20312845 著者名 Seino, Ken-Ichiro
言語	英語
発行日	2020-01-14
出版者	Nature Publishing Group
収録物名	Scientific reports
巻(号)	10(1)
ページ	224
抄録	The thymus plays a significant role in establishing immunological self-tolerance. Previous studies have revealed that host immune reaction to allogeneic transplants could be regulated by thymus transplantation. However, physiological thymus involution hinders the clinical application of these insights. Here, we report an efficient generation of thymic epithelial-like tissue derived from induced pluripotent stem cells (iPSCs) and its potential to regulate immune reaction in allogeneic transplantation. We established an iPSC line which constitutively expresses mouse Foxn1 gene and examined the effect of its expression during in vitro differentiation of thymic epithelial cells (TECs). We found that Foxn1 expression enhances the differentiation induction of cells expressing TEC-related cell surface molecules along with upregulation of endogenous Foxn1. iPSC-derived TECs (iPSC-TECs) generated T cells in nude recipient mice after renal subcapsular transplantation. Moreover, iPSC-TEC transplantation to immuno-competent recipients significantly prolonged the survival of allogeneic skin. Our study provides a novel concept for allogeneic transplantation in the setting of regenerative medicine.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2045-2322
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1038/s41598-019-57088-1