Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling

Yonamine, Yusuke; Hiramatsu, Kotaro; Ideguchi, Takuro et al.

RSC advances, 2020, 10(28), 16679-16686

Permalink : https://hdl.handle.net/2115/79511

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https://doi.org/10.1039/d0ra02803g     67

論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/79511
タイトル
Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling
著者
Yonamine, Yusuke ; Hiramatsu, Kotaro ; Ideguchi, Takuro ; Ito, Takuro ; Fujiwara, Tomomi ; Miura, Yoshiko ; Goda, Keisuke ; Hoshino, Yu
e-Rad_Researcher
50722716
著者名
Yonamine, Yusuke
著者名
Hiramatsu, Kotaro
著者名
Ideguchi, Takuro
著者名
Ito, Takuro
著者名
Fujiwara, Tomomi
著者名
Miura, Yoshiko
著者名
Goda, Keisuke
著者名
Hoshino, Yu
言語
英語
発行日
2020-04-28
出版者
Royal Society of Chemistry
収録物名
RSC advances
巻(号)
10(28)
ページ
16679-16686
抄録
Cellular metabolites are valuable in a diverse range of applications. For example, the unicellular green algaHaematococcus lacustrisproduces as a secondary metabolite the carotenoid pigment astaxanthin (AXT), which is widely used in nutraceutical, cosmetic, and food industries due to its strong antioxidant activity. In order to enhance the productivity ofH. lacustris, spatial and temporal understanding of its metabolic dynamics is essential. Here we show spatiotemporal monitoring of AXT production inH. lacustriscells by resonance Raman microscopy combined with stable isotope labeling. Specifically, we incorporated carbon dioxide ((CO2)-C-13) labeled with a stable isotope (C-13) intoH. lacustriscells through carbon fixation and traced its conversion to(13)C-AXT using our resonance Raman microscope. We incubatedH. lacustriscells under various conditions by switching, pulsing, and replacing(13)CO(2)and(12)CO(2). By measurement of these cells we determined the fixation time of(13)C-carbon, visualized the intracellular localization of(13)C- and(12)C-AXTs, and revealed the dynamic consumption-production equilibrium of the accumulated AXT. This work is a valuable step in the development of effective screening criteria for high AXT-producingH. lacustriscells.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
権利情報
Creative Commons Attribution-NonCommercial 3.0 Unported
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1039/d0ra02803g