open access

https://hdl.handle.net/2115/79521

Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats

英語

histochemistry

2019-10-09

Oxford University Press

Microscopy

68(5)

349-358

In this study, we aimed to clarify the role of ascorbic acid in collagen synthesis in periodontal ligaments using osteogenic disorder Shionogi (ODS)/ShiJcl-od/od rats lacking L-gulonolactone oxidase. These rats cannot synthesize ascorbic acid in vivo. Eight-week-old ODS/ShiJcl-od/od male rats were administered ascorbic acid solution at a concentration of 200 mg/dL (control group, n = 6) or ascorbic acid solution at concentration of 0.3 mg/dL (insufficient group, n = 12). Six rats of the insufficient group were then given with ascorbic acid solution at concentration of 200 mg/dL for additional 3 weeks (rescued group, n = 6), and then, their mandibles were histochemically examined. Consequently, the insufficient group specimens were seen to possess fewer collagen fibers, and silver impregnation revealed numerous fine, reticular fiber-like fibrils branching off from collagen in the periodontal ligaments. In control group, faint immunoreactivities for matrix metalloproteinase (MMP)2 and cathepsin H were seen in the periphery of blood vessels and throughout the ligament, respectively. In contrast, in the insufficient group, intense MMP2-immunoreactivity was observed to be associated with collagen fibrils in the periodontal ligaments, and cathepsin H-immunopositivity was seen in ligamentous cells. The rescued group showed abundant collagen fibers filling the periodontal ligament space. Under transmission electron microscopy, ligamentous fibroblasts incorporated collagen fibrils into tubular endosomes/lysosomes while simultaneously synthesizing collagen fibril bundles. Thus, ascorbic acid insufficiency affected the immunolocalization of cathepsin H and MMP2; however, ligamentous fibroblasts appear to possess the potential to synthesize collagen fibers when supplied with ascorbic acid.

学術雑誌論文

Accepted Manuscript

This is a pre-copyedited, author-produced version of an article accepted for publication in [Microscopy] following peer review. The version of record [Hasegawa, Tomoka; Miyamoto-Takasaki, Yukina; Abe, Miki; Qiu, Zixuan; Yamamoto, Tomomaya; Yimin; Yoshida, Taiji; Yoshino, Hirona; Hongo, Hiromi; Yokoyama, Ayako; Sasaki, Muneteru; Kuroshima, Shinichiro; Hara, Kuniko; Kobayashi, Masatoshi; Akiyama, Yasuhiro; Maeda, Takeyasu; Luiz de Freitas, Paulo Henrique; Li, Minqi; Amizuka, Norio; Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats. Microscopy, Volume 68, Issue 5, 9 October 2019, Pages 349–358] is available online at: [https://doi.org/10.1093/jmicro/dfz021].

2050-5698