Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification

Fukumoto, Tatsuya; Iwasaki, Sumio; Fujisawa, Shinichi; Hayasaka, Kasumi; Sato, Kaori; Oguri, Satoshi; Taki, Keisuke; Nakakubo, Sho; Kamada, Keisuke; Yamashita, Yu; Konno, Satoshi; Nishida, Mutsumi; Sugita, Junichi; Teshima, Takanori

Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification

Fukumoto, Tatsuya; Iwasaki, Sumio; Fujisawa, Shinichi et al.

International journal of infectious diseases, 2020, 98, 16-17

Permalink : https://hdl.handle.net/2115/79584

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アクセス権	open access
URI	https://hdl.handle.net/2115/79584
タイトル	Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification
著者	Fukumoto, Tatsuya ; Iwasaki, Sumio ; Fujisawa, Shinichi ; Hayasaka, Kasumi ; Sato, Kaori ; Oguri, Satoshi ; Taki, Keisuke ; Nakakubo, Sho ; Kamada, Keisuke ; Yamashita, Yu ; Konno, Satoshi ; Nishida, Mutsumi ; Sugita, Junichi ; Teshima, Takanori
著者名 Fukumoto, Tatsuya
著者名 Iwasaki, Sumio
著者名 Fujisawa, Shinichi
著者名 Hayasaka, Kasumi
著者名 Sato, Kaori
著者名 Oguri, Satoshi
著者名 Taki, Keisuke
著者名 Nakakubo, Sho
e-Rad_Researcher 80885666 著者名 Kamada, Keisuke
著者名 Yamashita, Yu
e-Rad_Researcher 20399835 著者名 Konno, Satoshi
e-Rad_Researcher 90404722 著者名 Nishida, Mutsumi
e-Rad_Researcher 70755694 著者名 Sugita, Junichi
e-Rad_Researcher 40284096 著者名 Teshima, Takanori
言語	英語
キーワード	COVID-19
	SARS-CoV-2
	PCR
	Saliva
発行日	2020-09
出版者	Elsevier
収録物名	International journal of infectious diseases
巻(号)	98
ページ	16-17
抄録	Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time byeliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	1201-9712
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.ijid.2020.06.074