Effects of the organochlorine p,p '-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes

Thompson, Lesa A.; Ikenaka, Yoshinori; Darwish, Wageh Sobhy; Nakayama, Shouta M. M.; Mizukawa, Hazuki; Ishizuka, Mayumi

Effects of the organochlorine p,p '-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes

Thompson, Lesa A.; Ikenaka, Yoshinori; Darwish, Wageh Sobhy et al.

Environmental toxicology and pharmacology, 2019, 72, UNSP 103249

Permalink : https://hdl.handle.net/2115/79687

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アクセス権	open access
URI	https://hdl.handle.net/2115/79687
タイトル	Effects of the organochlorine p,p '-DDT on MCF-7 cells: Investigating metabolic and immune modulatory transcriptomic changes
著者	Thompson, Lesa A. ; Ikenaka, Yoshinori ; Darwish, Wageh Sobhy ; Nakayama, Shouta M. M. ; Mizukawa, Hazuki ; Ishizuka, Mayumi
著者名 Thompson, Lesa A.
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
著者名 Darwish, Wageh Sobhy
e-Rad_Researcher 90647629 著者名 Nakayama, Shouta M. M.
e-Rad_Researcher 60612661 著者名 Mizukawa, Hazuki
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	p,p '-DDT
	MCF-7 cells
	Transcriptomics
	Biomarkers
発行日	2019-08-29
出版者	Elsevier
収録物名	Environmental toxicology and pharmacology
巻(号)	72
ページ	UNSP 103249
抄録	The organochlorine pesticide dichloro-diphenyl-trichloroethane (DDT) is persistent in the environment and leads to adverse human health effects. High levels in breast milk pose a threat to both breast tissue and nursing infants. The objectives of this study were to investigate DDT-induced transcriptomic alterations in enzymes and transporters involved in xenobiotic metabolism, immune responses, oxidative stress markers, and cell growth in a human breast cancer cell line. MCF-7 cells were exposed to both environmentally-relevant and previously-tested concentrations of p,p'-DDT in a short-term experiment. Significant up-regulation of metabolizing enzymes and transporters (ACHE, GSTO1, NQO1 and ABCC2) and oxidative stress markers (CXCL8, HMOX-1, NFE2L2 and TNF) was clearly observed. Conversely, UGT1A6, AHR and cell growth genes (FGF2 and VEGFA) were severely down-regulated. Identification of these genes helps to identify mechanisms of p,p'-DDT action within cells and may be considered as useful biomarkers for exposure to DDT contamination.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2019, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1382-6689
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.etap.2019.103249