Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines

Kawamoto, Masaki; Yamaji, Toshiyuki; Saito, Kyoko; Shirasago, Yoshitaka; Satomura, Kazuhiro; Endo, Toshinori; Fukasawa, Masayoshi; Hanada, Kentaro; Osada, Naoki

Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines

Kawamoto, Masaki; Yamaji, Toshiyuki; Saito, Kyoko et al.

Frontiers in Genetics, 2020, 11, 546106

Permalink : https://hdl.handle.net/2115/79830

このアイテムのアクセス数:700件（2026-08-26 16:49 集計）

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https://doi.org/10.3389/fgene.2020.546106			15

論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/79830
タイトル	Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines
著者	Kawamoto, Masaki ; Yamaji, Toshiyuki ; Saito, Kyoko ; Shirasago, Yoshitaka ; Satomura, Kazuhiro ; Endo, Toshinori ; Fukasawa, Masayoshi ; Hanada, Kentaro ; Osada, Naoki
著者名 Kawamoto, Masaki
著者名 Yamaji, Toshiyuki
著者名 Saito, Kyoko
著者名 Shirasago, Yoshitaka
著者名 Satomura, Kazuhiro
著者名 Endo, Toshinori
著者名 Fukasawa, Masayoshi
著者名 Hanada, Kentaro
e-Rad_Researcher 70416270 著者名 Osada, Naoki
言語	英語
キーワード	Huh7 cell line
	genome sequencing
	cell lines
	hepatitis C virus
	cell substrate
発行日	2020-10-09
出版者	Frontiers Media
収録物名	Frontiers in Genetics
巻(号)	11
ページ	546106
抄録	The human hepatoma-derived HuH-7 cell line and its derivatives (Huh7.5 and Huh7.5.1) have been widely used as a convenient experimental substitute for primary hepatocytes. In particular, these cell lines represent host cells suitable for propagating the hepatitis C virus (HCV) in vitro. The Huh7.5.1-8 cell line, a subline of Huh7.5.1, can propagate HCV more efficiently than its parental cells. To provide genomic information for cells' quality control, we performed whole-genome sequencing of HuH-7 and Huh7.5.1-8 and identified their characteristic genomic deletions, some of which are applicable to an in-house test for cell authentication. Among the genes related to HCV infection and replication, 53 genes were found to carry missense or loss-of-function mutations likely specific to the HuH-7 and/or Huh7.5.1-8. Eight genes, including DDX58 (RIG-I), BAX, EP300, and SPP1 (osteopontin), contained mutations observed only in Huh7.5.1-8 or mutations with higher frequency in Huh7.5.1-8. These mutations might be relevant to phenotypic differences between the two cell lines and may also serve as genetic markers to distinguish Huh7.5.1-8 cells from the ancestral HuH-7 cells.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3389/fgene.2020.546106