Expression and Localization of Paneth Cells and Their alpha-Defensins in the Small Intestine of Adult Mouse

Ohira, Shuya; Shinozaki, Ryuga; Nishida, Takuto et al.

Frontiers in Immunology, 2020, 11, 570296

Permalink : https://hdl.handle.net/2115/79842

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https://doi.org/10.3389/fimmu.2020.570296     13

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アクセス権
open access
URI
https://hdl.handle.net/2115/79842
タイトル
Expression and Localization of Paneth Cells and Their alpha-Defensins in the Small Intestine of Adult Mouse
著者
Ohira, Shuya ; Shinozaki, Ryuga ; Nishida, Takuto ; Kikuchi, Mani ; Ayabe, Tokiyoshi ; Nakamura, Kiminori ; Yokoi, Yuki ; Fukaya, Rie
著者名
Ohira, Shuya
著者名
Shinozaki, Ryuga
著者名
Nishida, Takuto
著者名
Kikuchi, Mani
著者名
Ayabe, Tokiyoshi
著者名
Nakamura, Kiminori
著者名
Yokoi, Yuki
著者名
Fukaya, Rie
言語
英語
キーワード
Paneth cell
alpha-defensin
cryptdin
Institute of Cancer Research mouse
innate immunity
germ-free mouse
発行日
2020-10
2020-10-13
出版者
Frontiers Media
収録物名
Frontiers in Immunology
巻(号)
11
ページ
570296
抄録
Paneth cells contribute to intestinal innate immunity by sensing bacteria and secreting alpha-defensin. In Institute of Cancer Research (ICR) mice, alpha-defensin termed cryptdin (Crp) in Paneth cells consists of six major isoforms, Crp1 to 6. Despite accumulating evidences that alpha-defensin functions in controlling the intestinal microbiota, topographical localization of Paneth cells in the small intestine in relation to functions of alpha-defensin remains to be determined. In this study, we examined the expression level of messenger RNA (mRNA) encoding six Crp-isoforms and Crp immunoreactivities using singly isolated crypts together with bactericidal activities of Paneth cell secretions from isolated crypts of duodenum, jejunum, and ileum. Here we showed that levels of Crp mRNAs in the single crypt ranged from 5 x 10(3) to 1 x 10(6) copies per 5 ng RNA. For each Crp isoform, the expression level in ileum was 4 to 50 times higher than that in duodenum and jejunum. Furthermore, immunohistochemical analysis of isolated crypts revealed that the average number of Paneth cell per crypt in the small intestine increased from proximal to distal, three to seven-fold, respectively. Both Crp1 and 4 expressed greater in ileal Paneth cells than those in duodenum or jejunum. Bactericidal activities in secretions of ileal Paneth cell exposed to bacteria were significantly higher than those of duodenum or jejunum. In germ-free mice, Crp expression in each site of the small intestine was attenuated and bactericidal activities released by ileal Paneth cells were decreased compared to those in conventional mice. Taken together, Paneth cells and their alpha-defensin in adult mouse appeared to be regulated topographically in innate immunity to control intestinal integrity.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
1664-3224
関連情報
PMID
33154750
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.3389/fimmu.2020.570296