Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis

Izumi, Hikari; Gen, Koichiro; Lokman, P. Mark; Hagihara, Seishi; Horiuchi, Moemi; Tanaka, Toshiomi; Ijiri, Shigeho; Adachi, Shinji

Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis

Izumi, Hikari; Gen, Koichiro; Lokman, P. Mark et al.

Molecular Reproduction and Development, 2019, 86(12), 1846-1864

Permalink : https://hdl.handle.net/2115/79855

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アクセス権	open access
URI	https://hdl.handle.net/2115/79855
タイトル	Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis
著者	Izumi, Hikari ; Gen, Koichiro ; Lokman, P. Mark ; Hagihara, Seishi ; Horiuchi, Moemi ; Tanaka, Toshiomi ; Ijiri, Shigeho ; Adachi, Shinji
e-Rad_Researcher 10784027 著者名 Izumi, Hikari
e-Rad_Researcher 80372051 著者名 Gen, Koichiro
著者名 Lokman, P. Mark
e-Rad_Researcher 80704501 著者名 Hagihara, Seishi
著者名 Horiuchi, Moemi
著者名 Tanaka, Toshiomi
e-Rad_Researcher 90425421 著者名 Ijiri, Shigeho
e-Rad_Researcher 40231930 著者名 Adachi, Shinji
言語	英語
キーワード	development
	Japanese eel
	maternal transcript
	qPCR
発行日	2019-09-23
出版者	John Wiley & Sons
収録物名	Molecular Reproduction and Development
巻(号)	86(12)
ページ	1846-1864
抄録	Our understanding of maternal control of development in vertebrates remains incomplete. In this study, we investigated levels of maternal transcripts in good and poor quality eggs from artificially matured Japanese eel, using RNA-Seq and quantitative polymerase chain reaction (qPCR), to identify candidate maternal transcripts related to development. De novo assembly or mapping of reads to the eel draft genome yielded 619,029 contigs and 85,906 transcripts, respectively; normalized read counts to these assemblies were calculated using reads (RPKM) or fragments (FPKM) per kilobase of transcript per million mapped reads. In silico screening identified 1,594 contigs and 150 transcripts with lower RPKM or FPKM in poor than in good quality eggs, 245 contigs, and 85 transcripts of which could be annotated by BLASTx, respectively. From selected contigs or transcripts, six genes (dnajb4, gnpat, card14, pdp1, fcgbp, ttn) had significantly lower messenger RNA levels in poor than in good quality eggs by qPCR. Multiple regression analysis showed that five genes (gnpat, b4galnt1, acsl6, rtkn, trim24) significantly correlated with hatchability. Taken together, 10 genes were identified as candidate maternal transcripts, regulating development in Japanese eel. Our results contribute to understanding the molecular basis for maternal control of development in vertebrates.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis, which has been published in final form at https://doi.org/10.1002/mrd.23273. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	1040-452X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/mrd.23273