The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti

Runtuwene, Lucky R.; Kawashima, Shuichi; Pijoh, Victor D.; Tuda, Josef S. B.; Hayashida, Kyoko; Yamagishi, Junya; Sugimoto, Chihiro; Nishiyama, Shoko; Sasaki, Michihito; Orba, Yasuko; Sawa, Hirofumi; Takasaki, Tomohiko; James, Anthony A.; Kobayashi, Takashi; Eshita, Yuki

The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti

Runtuwene, Lucky R.; Kawashima, Shuichi; Pijoh, Victor D. et al.

International Journal of Molecular Sciences, 2020, 21(20), 7520

Permalink : https://hdl.handle.net/2115/79916

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アクセス権	open access
URI	https://hdl.handle.net/2115/79916
タイトル	The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti
著者	Runtuwene, Lucky R. ; Kawashima, Shuichi ; Pijoh, Victor D. ; Tuda, Josef S. B. ; Hayashida, Kyoko ; Yamagishi, Junya ; Sugimoto, Chihiro ; Nishiyama, Shoko ; Sasaki, Michihito ; Orba, Yasuko ; Sawa, Hirofumi ; Takasaki, Tomohiko ; James, Anthony A. ; Kobayashi, Takashi ; Eshita, Yuki
著者名 Runtuwene, Lucky R.
著者名 Kawashima, Shuichi
著者名 Pijoh, Victor D.
著者名 Tuda, Josef S. B.
著者名 Hayashida, Kyoko
著者名 Yamagishi, Junya
著者名 Sugimoto, Chihiro
著者名 Nishiyama, Shoko
著者名 Sasaki, Michihito
著者名 Orba, Yasuko
著者名 Sawa, Hirofumi
著者名 Takasaki, Tomohiko
著者名 James, Anthony A.
著者名 Kobayashi, Takashi
e-Rad_Researcher 10082223 著者名 Eshita, Yuki
言語	英語
キーワード	lethal(2)-essential-for-life
	dengue
	Aedes aegypti
発行日	2020-10
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	21(20)
ページ	7520
抄録	Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post dengue infection to those of uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes a member of the heat shock 20 protein (HSP20) family, was upregulated following dengue virus type 2 (DENV-2) infection in vivo. The transcripts of this gene did not exhibit differential accumulation in mosquitoes exposed to insecticides or pollutants. The induction and overexpression of l(2)efl gene products using poly(I:C) resulted in decreased DENV-2 replication in the cell line. In contrast, the RNAi-mediated suppression of l(2)efl gene products resulted in enhanced DENV-2 replication, but this enhancement occurred only if multiple l(2)efl genes were suppressed. l(2)efl homologs induce the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2 alpha) in the fruit fly Drosophila melanogaster, and we confirmed this finding in the cell line. However, the mechanism by which l(2)efl phosphorylates eIF2 alpha remains unclear. We conclude that l(2)efl encodes a potential anti-dengue protein in the vector mosquito.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms21207520